In vivo toxicity of bioreactor-grown biomass and exopolysaccharides from Malaysian tiger milk mushroom mycelium for potential future health applications.

Autor: Usuldin SRA; Agro-Biotechnology Institute, Malaysia (ABI), National Institutes of Biotechnology Malaysia (NIMB), c/o HQ MARDI, 43400, Serdang, Selangor, Malaysia.; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia., Wan-Mohtar WAAQI; Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.; Bioresources and Bioprocessing Research Group, Institute of Biological Sciences, Faculty of Sciences, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.; Bioscience Research Institute, Athlone Institute of Technology, Athlone, Ireland., Ilham Z; Bioresources and Bioprocessing Research Group, Institute of Biological Sciences, Faculty of Sciences, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.; Environmental Science and Management Program, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia., Jamaludin AA; Environmental Science and Management Program, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia., Abdullah NR; Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200, Kuantan, Pahang, Malaysia., Rowan N; Bioscience Research Institute, Athlone Institute of Technology, Athlone, Ireland. nrowan@ait.ie.; Empower Eco Innovation Hub, Boora, Co. Offaly, Ireland. nrowan@ait.ie.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Nov 29; Vol. 11 (1), pp. 23079. Date of Electronic Publication: 2021 Nov 29.
DOI: 10.1038/s41598-021-02486-7
Abstrakt: Natural mycelial biomass (MB) and exopolysaccharides (EPS) of Malaysian tiger milk mushroom Lignosus rhinocerus are considered high-end components due to their high commercial potential value in drug discovery. This study aims to evaluate the toxicity of the mushroom extracts' generated in a bioreactor using the zebrafish embryo toxicity (ZFET) model assay as a new therapy for treating asthma. Both MB and EPS extracts, at concentrations 0.16-10 mg/mL, were tested for ZFET and early development effects on Zebrafish Embryos (ZE) during 24-120 h post-fertilisation (HPF). Findings revealed that MB was deemed safe with an LC 50 of 0.77 mg/mL; the EPS were non-toxic (LC 50 of 0.41 mg/mL). Neither MB nor EPS delayed hatching nor teratogenic defects in the treated ZE at a 2.5 mg/mL dose. There were no significant changes in the ZE heart rate after treatments with MB (130 beats/min) and EPS (140 beats/min), compared to that of normal ZE (120-180 beats/min). Mixing both natural compounds MB and EPS did not affect toxicity using ZFET testing; thus, intimating their safe future use as therapeutic interventions. This represents the first study to have used the ZFET assay on MB and EPS extracts of L. rhinocerus for future health applications.
(© 2021. The Author(s).)
Databáze: MEDLINE
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