Implementation of a prospective study for enhancing surveillance of invasive bacterial infections in North Africa.

Autor:	Smaoui H; University of Tunis El Manar, Faculty of Medicine of Tunis; Children's Hospital Béchir Hamza of Tunis, Laboratory of Microbiology, Tunis, Tunisia., Tali-Maamar H; Université d'Alger 1; Institut Pasteur of Algeria, Algiers, Algeria., Zouhair S; Faculty of Medicine and Pharmacy of Marrakech, University of Cadi Ayyad, Marrakech, Morocco., Bouheraoua S; Université d'Alger 1; Institut Pasteur of Algeria, Algiers, Algeria., Mefteh K; University of Tunis El Manar, Faculty of Medicine of Tunis; Children's Hospital Béchir Hamza of Tunis, Laboratory of Microbiology, Tunis, Tunisia., Bouskraoui M; Faculty of Medicine and Pharmacy of Marrakech, University of Cadi Ayyad, Marrakech, Morocco; Hospital of Mère-Enfant, Teaching hospital of Mohammed VI Marrakech, Marrakech, Morocco., Amiche A; Sanofi Pasteur, Dubai, United Arab Emirates., Khris M; Sanofi Pasteur, Casablanca, Morocco., Deghmane AE; Institut Pasteur, Paris, France., Taha MK; Institut Pasteur, Paris, France. Electronic address: mktaha@pasteur.fr.
Jazyk:	angličtina
Zdroj:	International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2022 Feb; Vol. 115, pp. 101-105. Date of Electronic Publication: 2021 Nov 27.
DOI:	10.1016/j.ijid.2021.11.036
Abstrakt:	Objectives: We implemented a project named MENINGSTOP in three countries of North Africa (Algeria, Morocco and Tunisia). The main objective was to use real-time PCR to detect, identify and type the three main agents (Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae) responsible for invasive bacterial infections (IBI). Methods: The protocol of WHO and US CDC for real-time PCR was used to detect and type the three bacterial agents in clinical samples. We also designated two quality exercises using an external interlaboratory study and cross-testing of 10% of randomly selected samples. Results: Among the 752 samples tested, 18% were positive for one of the three agents. N. meningitidis was the most frequent globally reaching 9% of all samples (7% to 17% range) followed by S. pneumoniae 8% of all samples (6% to 15%). Group B meningococci was the most frequent (74% of all positive samples for meningococci and ranging from 50% to 90%). Quality assurance showed >85% correlation scores. Conclusions: Real-time PCR can help improving epidemiological surveillance. Data confirm the prevalence of meningococci B. Our project adds a reliable tool to enhance surveillance and to help decision making in vaccination strategies against IBI. Competing Interests: Declaration of Competing Interest This collaborative work was in part funded by Sanofi Pasteur but the latter was not responsible for data collection, data interpretation, and writing of the report. The corresponding author, MKT, had full access to all the data in the study and had final responsibility for the decision to submit for publication. MKT also performs contract works for the Institut Pasteur funded by GSK and Pfizer outside the submitted manuscript, and MKT and AED have a patent with GSK, 630133. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject. AA and MK are Sanofi employees and may hold shares and/or stock options in the company. (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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