Diluent Containing Dimethylformamide Added With Sucrose Improves In Vitro Quality After Freezing/Thawing Stallion Sperm.

Autor: Moura TCM; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Arruda LCP; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. Electronic address: luciacpa@hotmail.com., Araújo Silva RAJ; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Silva RPF; Enzymology Laboratory (LABENZ), Department of Biochemistry, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Oliveira AS; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Tobal LFM; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Batista AM; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Carneiro GF; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil., Guerra MMP; Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2022 Feb; Vol. 109, pp. 103825. Date of Electronic Publication: 2021 Nov 26.
DOI: 10.1016/j.jevs.2021.103825
Abstrakt: The aim of this study was to investigate the effects of sucrose on post-thawed equine semen quality. Semen samples (n = 24) were collected from six stallions. They were diluted (200 × 10 6 sperm/mL) in a freezing medium based on skimmed milk, egg yolk, dimethylformamide, and supplemented with sucrose at concentrations of 0 (Control), 25, 50, and 100 mM and in a commercial extender (BotuCrio). Subsequently, they were filled in straws (0.5 mL) and subjected to freezing and storage (-196°C). Immediately after thawing (37°C, 30 seconds), semen samples were evaluated for kinetics (CASA), plasma and acrosomal membrane integrity, and mitochondrial membrane potential (flow cytometry). The addition of 50 and 100mM sucrose to the freezing extender increased (P < .05) the parameters of TM, PM, VCL, VSL, and VAP, compared to the control group. The WOB parameter of the group supplemented with 100 mM sucrose was higher (P < .05) than the control group. Higher values ​​(P < .05) of ALH and BCF were observed in groups treated with sucrose (25, 50, and 100 mM), compared to BotuCrio. The semen frozen in the presence of 100 mM sucrose presented higher percentages (P < .05) of sperm with intact plasma and acrosomal membranes, and high mitochondrial membrane potential in relation to the other groups. It is concluded that the addition of sucrose to equine semen freezing extender increase motility (50 and 100 mM), plasma and acrosomal membrane integrity preserve, and high sperm mitochondrial membrane potential (100 mM) after thawing.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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