Synthetic spatial patterning in bacteria: advances based on novel diffusible signals.

Autor: Oliver Huidobro M; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Tica J; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Wachter GKA; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Isalan M; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2022 Jun; Vol. 15 (6), pp. 1685-1694. Date of Electronic Publication: 2021 Nov 29.
DOI: 10.1111/1751-7915.13979
Abstrakt: Engineering multicellular patterning may help in the understanding of some fundamental laws of pattern formation and thus may contribute to the field of developmental biology. Furthermore, advanced spatial control over gene expression may revolutionize fields such as medicine, through organoid or tissue engineering. To date, foundational advances in spatial synthetic biology have often been made in prokaryotes, using artificial gene circuits. In this review, engineered patterns are classified into four levels of increasing complexity, ranging from spatial systems with no diffusible signals to systems with complex multi-diffusor interactions. This classification highlights how the field was held back by a lack of diffusible components. Consequently, we provide a summary of both previously characterized and some new potential candidate small-molecule signals that can regulate gene expression in Escherichia coli. These diffusive signals will help synthetic biologists to successfully engineer increasingly intricate, robust and tuneable spatial structures.
(© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje