A new method to localise and quantify oxidative stress in live juvenile mussels.

Autor: Delorme NJ; Aquaculture Group, Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand., Schmidt AJ; Hugh Green Cytometry Centre, Malaghan Institute of Medical Research, PO Box 7060, Wellington 6242, New Zealand., Zamora LN; Aquaculture Group, Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand., Burritt DJ; Botany Department, University of Otago, PO Box 56, Dunedin 9054, New Zealand., Ragg NLC; Aquaculture Group, Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand.
Jazyk: angličtina
Zdroj: Biology open [Biol Open] 2021 Dec 15; Vol. 10 (12). Date of Electronic Publication: 2021 Dec 14.
DOI: 10.1242/bio.059030
Abstrakt: Stress and survival of the juvenile New Zealand green-lipped mussel, Perna canaliculus, is a poorly understood bottleneck in the ecological and economic performance of a significant aquaculture crop. This species was therefore selected as a model organism for the development of a new method to quantify oxidative stress in whole individuals. An in vivo ROS-activated stain (CellROX™) was administered to anaesthetised, translucent juveniles that were subsequently formaldehyde fixed and then visualised using confocal microscopy. Subsequent application of image analysis to quantifying ROS-positive tissue areas was successfully used to detect stress differences in juvenile mussels exposed to varying levels of emersion. This integrated method can be used to localise and quantify ROS production in individual translucent bivalve life stages (larval and juvenile), while relative stability following fixation greatly expands potential practical field applications. This article has an associated First Person interview with the first and third authors of the paper.
Competing Interests: Competing interests The authors declare no competing or financial interests.
(© 2021. Published by The Company of Biologists Ltd.)
Databáze: MEDLINE