Characterization of the blood microbiota in children with Celiac disease.

Autor:	Mehrotra I; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA., Serena G; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA.; Mucosal Immunology and Biology Research Center, MassGeneral Hospital for Children, Jackson, 55 Fruit Street, Boston, MA 02114, USA.; Celiac Research Program, Harvard Medical School, Boston, MA, USA., Cetinbas M; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Department of Molecular Biology, Massachusetts General Hospital, Simches Research Center, 185 Cambridge Street, CPZN7250 Boston, MA 02114, USA., Kenyon V; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA.; Mucosal Immunology and Biology Research Center, MassGeneral Hospital for Children, Jackson, 55 Fruit Street, Boston, MA 02114, USA.; Celiac Research Program, Harvard Medical School, Boston, MA, USA., Martin VM; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Division of Pediatric Gastroenterology and Nutrition, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Yawkey 6B-6800 Suite, 55 Fruit Street Boston, MA 02114, USA.; Food Allergy Center, Massachusetts General Hospital, MGH Professional Office Building, Suite 530, 275 Cambridge Street Boston, MA 02114, USA., Harshman SG; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Eating Disorders Clinical and Research Program, Massachusetts General Hospital: West End Apartments 2 Longfellow Place, Suite 200 Boston, MA 02114, USA.; Neuroendocrine Unit, Massachusetts General Hospital, 100 Blossom St, Boston, MA 02114, USA., Zomorrodi AR; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA.; Mucosal Immunology and Biology Research Center, MassGeneral Hospital for Children, Jackson, 55 Fruit Street, Boston, MA 02114, USA.; Celiac Research Program, Harvard Medical School, Boston, MA, USA.; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA., Sadreyev RI; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Department of Molecular Biology, Massachusetts General Hospital, Simches Research Center, 185 Cambridge Street, CPZN7250 Boston, MA 02114, USA., Fasano A; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA.; Mucosal Immunology and Biology Research Center, MassGeneral Hospital for Children, Jackson, 55 Fruit Street, Boston, MA 02114, USA.; Celiac Research Program, Harvard Medical School, Boston, MA, USA.; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Division of Pediatric Gastroenterology and Nutrition, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Yawkey 6B-6800 Suite, 55 Fruit Street Boston, MA 02114, USA., Leonard MM; Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Suite 6B, 32 Fruit Street, Boston, MA 02114, USA.; Mucosal Immunology and Biology Research Center, MassGeneral Hospital for Children, Jackson, 55 Fruit Street, Boston, MA 02114, USA.; Celiac Research Program, Harvard Medical School, Boston, MA, USA.; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.; Division of Pediatric Gastroenterology and Nutrition, MassGeneral Hospital for Children, Yawkey Center for Outpatient Care, Yawkey 6B-6800 Suite, 55 Fruit Street Boston, MA 02114, USA.
Jazyk:	angličtina
Zdroj:	Current research in microbial sciences [Curr Res Microb Sci] 2021 Aug 30; Vol. 2, pp. 100069. Date of Electronic Publication: 2021 Aug 30 (Print Publication: 2021).
DOI:	10.1016/j.crmicr.2021.100069
Abstrakt:	Celiac Disease (CD) is an autoimmune disorder triggered by gluten ingestion that can develop in genetically predisposed individuals. Alterations in the gut microbiota have been suggested to contribute to development of autoimmune conditions including CD. Recent work suggests the existence of a blood microbiota. Evidence that alterations in the blood microbiota potentially influence the development of chronic immune based diseases is increasing. However, there is no published literature regarding the blood microbiota in children, including those with CD. This study aimed to characterize the diversity and taxonomic composition of the blood microbiota of children with CD compared to controls. Whole blood samples were collected from children with active CD, CD in remission, and control subjects and 16S rRNA sequencing was utilized to analyze the blood microbiota. We found 16s rRNA present throughout all pediatric blood samples, providing evidence for the presence of a pediatric blood microbiota. We found significant differences in beta diversity and in abundance of certain taxa (Campylobacterales order, Odoribacteraceae and Helicobacteraceae families, Odoribacter genus and species, and Bacteroides acidifaciens species) between subjects with active CD and controls. These taxa have been previously reported to be associated with immune response and gut-inflammatory diseases. We did not find significant differences between subjects with active and remission CD or between remission CD and controls. Conclusions: We provide evidence for a pediatric blood microbiota and identified higher beta diversity and alterations in the composition of blood microbiota in subjects with active CD compared to controls. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Alessio Fasano reports a relationship with Alba Therapeutics that includes: equity or stocks. Alessio Fasano reports a relationship with Innovate Biopharmaceuticals that includes: consulting or advisory. Alessio Fasano reports a relationship with Axial Biotherapeutics that includes: board membership. Alessio Fasano reports a relationship with Viome that includes: board membership. Alessio Fasano reports a relationship with Mead Johnson Nutrition that includes: speaking and lecture fees. Maureen M Leonard reports a relationship with Anokion that includes: consulting or advisory. Maureen M Leonard reports a relationship with 9meters biopharma that includes: board membership. Victoria M Martin reports a relationship with Milk Care Co that includes: board membership. (© 2021 The Authors. Published by Elsevier B.V.)
Databáze:	MEDLINE
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