Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt.

Autor: Hassanien RT; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Hamdy ME; Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Elnomrosy SM; Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Hussein HA; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Afify AF; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Darwish FM; Department of Pathology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Shehab G; Department of Pathology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Emran R; Department of Pathology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Abd-El-Moniem MII; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Habashi AR; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Fahmy HA; Department of Biotechnology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Ibraheem EM; Department of Pathology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Shahein MA; Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt., Attya M; General Organization of Veterinary Services, Dokki, Giza, Egypt., Abdelhakim AMM; General Organization of Veterinary Services, Dokki, Giza, Egypt., Hagag NM; Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2021 Sep; Vol. 14 (9), pp. 2296-2305. Date of Electronic Publication: 2021 Sep 03.
DOI: 10.14202/vetworld.2021.2296-2305
Abstrakt: Background and Aim: Bovine papillomaviruses (BPV) are a heterogeneous group of oncoviruses, distributed globally, which produce major economic losses. In the current study, we compared the results of different diagnostic approaches and compared the strains identified in this study with previously characterized strains at local and international levels.
Materials and Methods: Samples of skin warts were collected from five bovines with generalized papillomatosis from two Egyptian provinces, Menya and Ismailia, in 2020. Electron microscopy, molecular characterization, histopathological, and immunohistochemical examination were performed.
Results: BPV was detected using electron microscopy in the collected samples. Using molecular characterization, BPV-2 was successfully identified for 1 st time in Egypt. The strain has 99.6% identity with the BPV-2 reference strains obtained from GenBank. These results were supported by histopathology and immunohistochemistry examination. Partial nucleotide sequences of the L1 gene were submitted to GenBank with accession numbers MW289843 and MW289844.
Conclusion: BPV-2 was reported for 1 st time in the current study. The strain was identified grossly, microscopically, and pathologically and confirmed using molecular approaches. All results were consistent. The sequence analysis revealed that this strain has high sequence similarity to the reference Deltapapillomavirus-4, BPV-2 strains from Brazil and China.
(Copyright: © Hassanien, et al.)
Databáze: MEDLINE
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