Effects of Resistant Starch on Symptoms, Fecal Markers, and Gut Microbiota in Parkinson's Disease - The RESISTA-PD Trial.

Autor:	Becker A; Department of Neurology, Saarland University, D-66421 Homburg, Germany., Schmartz GP; Chair for Clinical Bioinformatics, Saarland University, D-66123 Saarbrücken, Germany., Gröger L; Department of Human Genetics, Saarland University, D-66421 Homburg, Germany., Grammes N; Chair for Clinical Bioinformatics, Saarland University, D-66123 Saarbrücken, Germany., Galata V; Chair for Clinical Bioinformatics, Saarland University, D-66123 Saarbrücken, Germany., Philippeit H; Department of Neurology, Saarland University, D-66421 Homburg, Germany., Weiland J; Department of Neurology, Saarland University, D-66421 Homburg, Germany., Ludwig N; Department of Human Genetics, Saarland University, D-66421 Homburg, Germany., Meese E; Department of Human Genetics, Saarland University, D-66421 Homburg, Germany., Tierling S; Department of Genetics/Epigenetics, Saarland University, D-66123 Saarbrücken, Germany., Walter J; Department of Genetics/Epigenetics, Saarland University, D-66123 Saarbrücken, Germany., Schwiertz A; Institute of Microecology, D-35745 Herborn, Germany., Spiegel J; Department of Neurology, Saarland University, D-66421 Homburg, Germany., Wagenpfeil G; Institute of Medical Biometry, Epidemiology and Medical Informatics, Saarland University, D-66421 Homburg, Germany., Faßbender K; Department of Neurology, Saarland University, D-66421 Homburg, Germany., Keller A; Chair for Clinical Bioinformatics, Saarland University, D-66123 Saarbrücken, Germany; Department of Neurology, Stanford University, Palo Alto, CA 94305, USA., Unger MM; Department of Neurology, Saarland University, D-66421 Homburg, Germany. Electronic address: marcus.unger@uks.eu.
Jazyk:	angličtina
Zdroj:	Genomics, proteomics & bioinformatics [Genomics Proteomics Bioinformatics] 2022 Apr; Vol. 20 (2), pp. 274-287. Date of Electronic Publication: 2021 Nov 25.
DOI:	10.1016/j.gpb.2021.08.009
Abstrakt:	The composition of the gut microbiota is linked to multiple diseases, including Parkinson's disease (PD). Abundance of bacteria producing short-chain fatty acids (SCFAs) and fecal SCFA concentrations are reduced in PD. SCFAs exert various beneficial functions in humans. In the interventional, monocentric, open-label clinical trial "Effects of Resistant Starch on Bowel Habits, Short Chain Fatty Acids and Gut Microbiota in Parkinson'sDisease" (RESISTA-PD; ID: NCT02784145), we aimed at altering fecal SCFAs by an 8-week prebiotic intervention with resistant starch (RS). We enrolled 87 subjects in three study-arms: 32 PD patients received RS (PD + RS), 30 control subjects received RS, and 25 PD patients received solely dietary instructions. We performed paired-end 100 bp length metagenomic sequencing of fecal samples using the BGISEQ platform at an average of 9.9 GB. RS was well-tolerated. In the PD + RS group, fecal butyrate concentrations increased significantly, and fecal calprotectin concentrations dropped significantly after 8 weeks of RS intervention. Clinically, we observed a reduction in non-motor symptom load in the PD + RS group. The reference-based analysis of metagenomes highlighted stable alpha-diversity and beta-diversity across the three groups, including bacteria producing SCFAs. Reference-free analysis suggested punctual, yet pronounced differences in the metagenomic signature in the PD + RS group. RESISTA-PD highlights that a prebiotic treatment with RS is safe and well-tolerated in PD. The stable alpha-diversity and beta-diversity alongside altered fecal butyrate and calprotectin concentrations call for long-term studies, also investigating whether RS is able to modify the clinical course of PD. (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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