Circulating plasma miR-155 is a potential biomarker for the detection of SARS-CoV-2 infection.

Autor: Haroun RA; Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. Electronic address: rihamharoun8@gmail.com., Osman WH; Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt., Amin RE; Department of Clinical and Chemical Pathology, Faculty of Medicine, Port-Said University, Port-Said, Egypt., Hassan AK; Department of Zoology, Faculty of Science, Port-Said University, Port-Said, Egypt., Abo-Shanab WS; Department of Radiodiagnosis, Faculty of Medicine, Port-Said University, Port-Said, Egypt., Eessa AM; Department of Geriatric Medicine and Gerontology, Faculty of Medicine, Port-Said University, Port-Said, Egypt.
Jazyk: angličtina
Zdroj: Pathology [Pathology] 2022 Feb; Vol. 54 (1), pp. 104-110. Date of Electronic Publication: 2021 Nov 06.
DOI: 10.1016/j.pathol.2021.09.006
Abstrakt: Identification of human miRNAs involved in coronavirus-host interplay is important due to the current COVID-19 pandemic. Therefore, this study aimed to measure the circulating plasma miR-155 expression level in COVID-19 patients and healthy controls to investigate its roles in the pathogenesis and severity of COVID-19 disease and to assess its usefulness as a clinical biomarker for the detection of COVID-19 disease and the severity of infection. A total of 150 COVID-19 patients and 50 controls were enrolled into our study. Beside the routine laboratory work and chest computed tomography (CT) scans of COVID-19 patients, plasma miR-155 expression level was measured using reverse transcription quantitative real-time PCR (RT-qPCR) technique. Our results demonstrated increased miR-155 expression level in COVID-19 patients compared to controls, in severe compared to moderate COVID-19 patients, and in non-survival compared to survival COVID-19 patients. miR-155 expression level also had significant correlation with clinicopathological characteristics of COVID-19 patients such as chest CT findings, CRP, ferritin, mortality, D-dimer, WBC count, and lymphocytes and neutrophils percentages. Also, our results showed that the area under the curve (AUC) for miR-155 was 0.986 with 90% sensitivity and 100% specificity when used as a biomarker for the detection of COVID-19 disease; while in detection of severity of COVID-19 disease, AUC for miR-155 was 0.75 with 76% sensitivity and specificity. From these results we can conclude that miR-155 has a crucial role in the pathogenesis and severity of COVID-19; also, it could be a good diagnostic clinical biomarker for the detection of COVID-19 disease and the severity of infection.
(Copyright © 2021 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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