| Autor: |
Silva RN; Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA., Zhang P; Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA. zhangph@ucmail.uc.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2393, pp. 611-622. |
| DOI: |
10.1007/978-1-0716-1803-5_32 |
| Abstrakt: |
Nuclear relaxation-based techniques rely on the capability of paramagnetic center to affect the transverse relaxation time (T 2 ) of the water protons. These changes are sensitive to the microenvironment of the paramagnetic center, which can be used to detect a variety of targets. In this work, we present an "on-off" oligonucleotide detection scheme in aqueous solutions, which uses gadolinium phthalocyanine (GdTcPc)-grafted silica nanoparticles as the paramagnetic centers. A probe oligonucleotide strand was conjugated to the GdTcPc to act as a recognition element. In the presence of the target oligonucleotide, which was complementary to the probe, an increase in the ΔT 2 value, which was measured by subtracting the characteristic T 2 value of the background solution from that of the sample using a benchtop relaxometer, was observed. The magnitude of this increase was proportional to the target oligonucleotide concentration. A linear range was obtained from 30 to 140 nM, with a detection limit of 15 nM. The developed nuclear relaxation-based detection scheme is shown to be a simple, fast, and selective method to detect oligonucleotide and could be useful in point-of-care diagnostic applications.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
