miR-1290 promotes IL-8-mediated vascular endothelial cell adhesion by targeting GSK-3β.

Autor: Xu H; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Cui Y; Molecular Medical Laboratory, College of Basic Medical Science, Dalian Medical University, Dalian, China.; Liaoning Provincial Core Lab of Medical Molecular Biology, Dalian Medical University, Dalian, China., Liu X; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Zheng X; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Liu J; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Hu X; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Gao F; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Hu X; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Li M; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China., Wei X; Molecular Medical Laboratory, College of Basic Medical Science, Dalian Medical University, Dalian, China.; Liaoning Provincial Core Lab of Medical Molecular Biology, Dalian Medical University, Dalian, China., Gao Y; Liaoning Provincial Core Lab of Medical Molecular Biology, Dalian Medical University, Dalian, China. gaoying200018@126.com.; Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian, China. gaoying200018@126.com., Zhao Y; Molecular Medical Laboratory, College of Basic Medical Science, Dalian Medical University, Dalian, China. zhaoying20001105@126.com.; Liaoning Provincial Core Lab of Medical Molecular Biology, Dalian Medical University, Dalian, China. zhaoying20001105@126.com.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2022 Mar; Vol. 49 (3), pp. 1871-1882. Date of Electronic Publication: 2021 Nov 27.
DOI: 10.1007/s11033-021-06998-3
Abstrakt: Background: MicroRNA-1290 (miR-1290) has been reported to be involved in many diseases and play a key role during the development process. However, the role of miR-1290 in atherosclerosis (AS) is still unclear.
Methods and Results: The current study showed that the expressions of miR-1290 were high in serum of patients with hyperlipidemia. The functional role of miR-1290 were then investigated in human umbilical vein endothelial cells (HUVECs). Here, we found that miR-1290 expressions were notably enhanced in HUVECs mediated by IL-8. miR-1290 inhibitor repressed monocytic THP-1 cells adhesion to HUVECs by regulating ICAM-1 and VCAM-1, inhibited proliferation through regulating cyclinD1 and PCNA, and inhibited inflammatory response by regulating IL-1β. Mechanistically, we verified that miR-1290 mimic was able to directly target the 3'-UTR of GSK-3β mRNA using luciferase reporter assay. Knockdown of GSK-3β (si-GSK-3β) promoted HUVECs adhesion and the expression of IL-1β, and partially restore the depression effect of miR-1290 inhibitor on HUVECs adhesion and inflammation. In contrast, si-GSK-3β inhibited the proliferation of HUVECs and the expression of cyclinD1 and PCNA.
Conclusions: In summary, our study revealed that miR-1290 promotes IL-8-mediated the adhesion of HUVECs by targeting GSK-3β. However, GSK-3β is not the target protein for miR-1290 to regulate the proliferation of HUVECs. Our findings may provide potential target in atherosclerosis treatment.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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