| Autor: |
Ramsay JA; Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada., de Lima E Silva MR; Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada., Tawadrous MAR; Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada., Ramsay BA; Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada. |
| Jazyk: |
angličtina |
| Zdroj: |
Microorganisms [Microorganisms] 2021 Oct 26; Vol. 9 (11). Date of Electronic Publication: 2021 Oct 26. |
| DOI: |
10.3390/microorganisms9112224 |
| Abstrakt: |
We examined greenhouse gas (GHG) production upon the addition of ammonium and phosphate to mature fine tailing (MFT) samples from Alberta's Pond 2/3 (at 5 and 15 m) and Pond 7 (12.5 m) in microcosm studies. The methane production rate in unamended Pond 2/3 MFT correlated with sample age; the production rate was higher in the less dense, more recently discharged MFT samples and lower in the denser, deeper sample. Adding small amounts of naphtha increased methane production, but there was no correlation with increasing naphtha, indicating that naphtha may partition into bitumen, reducing its bioavailability. Although non-detectable phosphate and low ammonium in the pore water indicate that these nutrients were potentially limiting microbial activity, their addition did not significantly affect methanogenesis but somewhat enhanced sulphate and nitrate reduction. Neither ammonium nor phosphate were detected in the pore water when added at low concentrations, but when added at high concentrations, 25-35% phosphate and 30-45% ammonium were lost. These ions likely sorbed to MFT minerals such as kaolinite, which have microbial activity governed by phosphate/ammonium desorption. Hence, multiple limitations affected microbial activity. Sulphate was less effective than nitrate was in inhibiting methanogenesis because H 2 S may be a less effective inhibitor than NO x - intermediates are, and/or H 2 S may be more easily abiotically removed. With nitrate reduction, N 2 O, a potent GHG was produced but eventually metabolized. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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