Mycorrhiza-Induced Alterations in Metabolome of Medicago lupulina Leaves during Symbiosis Development.

Autor: Yurkov AP; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Puzanskiy RK; Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia., Avdeeva GS; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Jacobi LM; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Gorbunova AO; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia.; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Kryukov AA; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Kozhemyakov AP; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Laktionov YV; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Kosulnikov YV; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Romanyuk DA; Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia., Yemelyanov VV; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Shavarda AL; Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia.; Center for Molecular and Cell Technologies, St. Petersburg State University, 19934 St. Petersburg, Russia., Kirpichnikova AA; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Smolikova GN; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Shishova MF; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Plants (Basel, Switzerland) [Plants (Basel)] 2021 Nov 18; Vol. 10 (11). Date of Electronic Publication: 2021 Nov 18.
DOI: 10.3390/plants10112506
Abstrakt: The present study is aimed at disclosing metabolic profile alterations in the leaves of the Medicago lupulina MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with Rhizophagus irregularis under condition of a low phosphorus level in the substrate. A highly effective AM symbiosis was established in the period from the stooling to the shoot branching initiation stage (the efficiency in stem height exceeded 200%). Mycorrhization led to a more intensive accumulation of phosphates (glycerophosphoglycerol and inorganic phosphate) in M. lupulina leaves. Metabolic spectra were detected with GS-MS analysis. The application of complex mathematical analyses made it possible to identify the clustering of various groups of 320 metabolites and thus demonstrate the central importance of the carbohydrate and carboxylate-amino acid clusters. The results obtained indicate a delay in the metabolic development of mycorrhized plants. Thus, AM not only accelerates the transition between plant developmental stages but delays biochemical "maturation" mainly in the form of a lag of sugar accumulation in comparison with non-mycorrhized plants. Several methods of statistical modeling proved that, at least with respect to determining the metabolic status of host-plant leaves, stages of phenological development have priority over calendar age.
Databáze: MEDLINE
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