| Autor: |
Abderrezag N; Laboratory of Environmental Process Engineering, Faculty of Process Engineering, University Salah Boubnider-Constantine 3, Constantine 25000, Algeria.; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), C/Nicolás Cabrera 9, 28049 Madrid, Spain., Sánchez-Martínez JD; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), C/Nicolás Cabrera 9, 28049 Madrid, Spain., Louaer O; Laboratory of Environmental Process Engineering, Faculty of Process Engineering, University Salah Boubnider-Constantine 3, Constantine 25000, Algeria., Meniai AH; Laboratory of Environmental Process Engineering, Faculty of Process Engineering, University Salah Boubnider-Constantine 3, Constantine 25000, Algeria., Mendiola JA; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), C/Nicolás Cabrera 9, 28049 Madrid, Spain. |
| Abstrakt: |
Ammodaucus leucotrichus is a spontaneous plant endemic of the North African region. An efficient selective pressurized liquid extraction (PLE) method was optimized to concentrate neuroprotective extracts from A. leucotrichus fruits. Green solvents were tested, namely ethanol and water, within a range of temperatures between 40 to 180 °C. Total carbohydrates and total phenolics were measured in extracts, as well as in vitro antioxidant capacity (DPPH radical scavenging), anticholinesterase (AChE) and anti-inflammatory (LOX) activities. Metabolite profiling was carried out by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-q-TOF-MS/MS), identifying 94 compounds. Multivariate analysis was performed to correlate composition with bioactivity. A remarkable effect of the temperature using water was observed: the higher temperature, the higher extraction yield, the higher total phenolic content, as well as the higher total carbohydrates content. The water extract obtained at 180 °C, 10.34 MPa and 10 min showed meaningful anti-inflammatory (IC50 LOX = 39.4 µg/mL) and neuroprotective activities (IC50 AChE = 55.6 µg/mL). The Principal Components Analysis (PCA) and the cluster analysis correlated these activities with the presence of carbohydrates and phenolic compounds. |
