The Membrane Electrical Potential and Intracellular pH as Factors Influencing Intracellular Ascorbate Concentration and Their Role in Cancer Treatment.

Autor: Gąbka M; Faculty of Fundamental Problem of Technology, Wroclaw University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 17, 50-370 Wrocław, Poland., Dałek P; Department of Biomedical Engineering, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 17, 50-370 Wrocław, Poland.; Lipid Systems Sp. z o.o., ul. Krzemieniecka 48c, 54-613 Wrocław, Poland., Przybyło M; Department of Biomedical Engineering, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 17, 50-370 Wrocław, Poland.; Lipid Systems Sp. z o.o., ul. Krzemieniecka 48c, 54-613 Wrocław, Poland., Gackowski D; Department of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Karłowicza 24, 85-092 Bydgoszcz, Poland., Oliński R; Department of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Karłowicza 24, 85-092 Bydgoszcz, Poland., Langner M; Department of Biomedical Engineering, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 17, 50-370 Wrocław, Poland.; Lipid Systems Sp. z o.o., ul. Krzemieniecka 48c, 54-613 Wrocław, Poland.
Jazyk: angličtina
Zdroj: Cells [Cells] 2021 Oct 30; Vol. 10 (11). Date of Electronic Publication: 2021 Oct 30.
DOI: 10.3390/cells10112964
Abstrakt: Ascorbate is an important element of a variety of cellular processes including the control of reactive oxygen species levels. Since reactive oxygen species are implicated as a key factor in tumorigenesis and antitumor therapy, the injection of a large amount of ascorbate is considered beneficial in cancer therapy. Recent studies have shown that ascorbate can cross the plasma membrane through passive diffusion. In contrast to absorption by active transport, which is facilitated by transport proteins (SVCT1 and SVCT2). The passive diffusion of a weak acid across membranes depends on the electrostatic potential and the pH gradients. This has been used to construct a new theoretical model capable of providing steady-state ascorbate concentration in the intracellular space and evaluating the time needed to reach it. The main conclusion of the analysis is that the steady-state intracellular ascorbate concentration weakly depends on its serum concentration but requires days of exposure to saturate. Based on these findings, it can be hypothesized that extended oral ascorbate delivery is possibly more effective than a short intravenous infusion of high ascorbate quantities.
Databáze: MEDLINE
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