| Autor: |
Danesi AL; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Athanasiadou D; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Mansouri A; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Phen A; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Neshatian M; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Holcroft J; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada., Bonde J; Division of Pure and Applied Biochemistry, Center of Applied Life Sciences, Lund University, 223 62 Lund, Sweden., Ganss B; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada.; Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada., Carneiro KMM; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada.; Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada. |
| Abstrakt: |
Biomineralization is a crucial process whereby organisms produce mineralized tissues such as teeth for mastication, bones for support, and shells for protection. Mineralized tissues are composed of hierarchically organized hydroxyapatite crystals, with a limited capacity to regenerate when demineralized or damaged past a critical size. Thus, the development of protein-based materials that act as artificial scaffolds to guide hydroxyapatite growth is an attractive goal both for the design of ordered nanomaterials and for tissue regeneration. In particular, amelogenin, which is the main protein that scaffolds the hierarchical organization of hydroxyapatite crystals in enamel, amelogenin recombinamers, and amelogenin-derived peptide scaffolds have all been investigated for in vitro mineral growth. Here, we describe uniaxial hydroxyapatite growth on a nanoengineered amelogenin scaffold in combination with amelotin, a mineral promoting protein present during enamel formation. This bio-inspired approach for hydroxyapatite growth may inform the molecular mechanism of hydroxyapatite formation in vitro as well as possible mechanisms at play during mineralized tissue formation. |
