TiO 2 -NPs and cadmium co-exposure: in vitro assessment of genetic and genomic DNA damage on Dicentrarchus labrax embryonic cells.

Autor: Mottola F; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy., Santonastaso M; Department of Woman, Child and General and Special Surgery, University of Campania 'Luigi Vanvitelli', Napoli, Italy., Iovine C; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy., Frenzilli G; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy., Picchietti S; Department of Innovation of Biological Systems, Food and Forestry DIBA, Tuscia University, Viterbo, Italy., Genualdo V; Laboratory of Animal Cytogenetics and Genomics, National Research Council (CNR), ISPAAM, P.le Enrico Fermi 1, Naples, Italy., Rocco L; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy. lucia.rocco@unicampania.it.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Sep; Vol. 29 (41), pp. 62208-62218. Date of Electronic Publication: 2021 Nov 26.
DOI: 10.1007/s11356-021-17645-6
Abstrakt: The increased titanium dioxide nanoparticles (TiO 2 -NPs) spread and their interaction with organic and inorganic pollutants arouses concern for the potential hazards for organisms and environment. This study tested in vitro the genotoxic effects of TiO 2 -NPs (1 μg/mL) and cadmium (Cd) (0.1 μg/mL) co-exposure using Dicentrarchus labrax embryonic cells (DLEC) as experimental model. The genotoxicity tests (Comet assay, Diffusion Assay and Random Amplification of Polymorphic DNA (RAPD-PCR) were conducted after 3, 24 and 48 hours of exposure to TiO 2 -NPs and Cd alone and in combination. The results showed that the percentage of DNA damage and apoptotic cells increases following 48 hours TiO 2 -NPs exposure, while DNA instability was detected for all the times tested. Cd induced genotoxic effects starting from 3 hour-exposure and for all the treatment times. Cd + TiO 2 -NPs co-exposure did not cause any genomic damage or apoptosis for all the exposure times. The possibility that Cd and TiO 2 -NPs form aggregates no longer able of penetrating the nucleus and damaging the genetic material is discussed.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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