"Q-markers targeted screening" strategy for comprehensive qualitative and quantitative analysis in fingerprints of Angelica dahurica with chemometric methods.

Autor:	Gao FY; Department of Health Toxicology, Faculty of Naval Medicine, Second Military Medical University, No. 800 Xiangyin Road, Shanghai 200433, China., Chen HY; Department of Endocrinology, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai 200003, China., Luo YS; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China., Chen JK; Department of Health Toxicology, Faculty of Naval Medicine, Second Military Medical University, No. 800 Xiangyin Road, Shanghai 200433, China., Yan L; Department of Health Toxicology, Faculty of Naval Medicine, Second Military Medical University, No. 800 Xiangyin Road, Shanghai 200433, China., Zhu JB; Department of Health Toxicology, Faculty of Naval Medicine, Second Military Medical University, No. 800 Xiangyin Road, Shanghai 200433, China., Fan GR; Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, No. 100 Haining Road, Shanghai 200025, China.; School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, China., Zhou TT; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.
Jazyk:	angličtina
Zdroj:	Food chemistry: X [Food Chem X] 2021 Nov 13; Vol. 12, pp. 100162. Date of Electronic Publication: 2021 Nov 13 (Print Publication: 2021).
DOI:	10.1016/j.fochx.2021.100162
Abstrakt:	Angelica dahurica is a famous functional food and herb. To guarantee quality of A. dahurica, a strategy "Q-markers targeted screening" was successfully developed by sufficient extraction of compounds and the targeted screening of qualitative and quantitative markers calculated through chemometric methods based fingerprints. Accelerated solvent extraction was selected due to its prominent advantages exhibiting the maximum extraction yields and varieties of compounds and especially excellent reproducibility (RSD < 1). After extraction, the fingerprints of A. dahuricae samples were established. For the preliminary herb authenticity, the targeted screening of 23 quantitative markers were performed by similarity analysis and hierarchical cluster analysis based on the fingerprints, which were identified by liquid chromatography tandem mass spectrometry (LC-MS). Subsequently, for further quality control, the targeted screening of nine quantitative markers were done by similarity analysis & linear discriminant analysis, which were determined by LC. Lastly, the strategy was successfully applied to quality assessment of A. dahurica samples. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 Published by Elsevier Ltd.)
Databáze:	MEDLINE
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