Antibacterial, remineralising and matrix metalloproteinase inhibiting scandium-doped phosphate glasses for treatment of dental caries.

Autor: Valappil SP; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK. Electronic address: S.Valappil@liverpool.ac.uk., Abou Neel EA; Preventive and Restorative Dentistry Department, College of Dental Medicine, University of Sharjah, 27272 Sharjah, United Arab Emirates; Biomaterials Department, Faculty of Dentistry, Tanta University, Tanta, Egypt; UCL, Eastman Dental Institute, Biomaterials and Tissue Engineering Division, Royal Free Hospital, UCL Medical School, Rowland Hill Street, Hampstead, London NW3 2PF, UK., Pickup DM; School of Physical Sciences, University of Kent, Canterbury CT2 7NH, UK., Burden E; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK., Sahdev R; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK., Miles EJ; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK., Cooper L; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK., Ansari TI; Northwick Park Institute for Medical Research, Watford Road, Harrow HA1 3UJ, UK., Hanna JV; Department of Physics, University of Warwick, Coventry CV4 7AL, UK., Higham SM; School of Dentistry, Institute of Life Course and Medical Sciences, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, UK.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2022 Jan; Vol. 38 (1), pp. 94-107. Date of Electronic Publication: 2021 Nov 22.
DOI: 10.1016/j.dental.2021.10.014
Abstrakt: Objectives: Antibiotic resistance is increasingly a growing global threat. This study aimed to investigate the potential use of newly developed scandium-doped phosphate-based glasses (Sc-PBGs) as an antibacterial and anticariogenic agent through controlled release of Sc 3+ ions.
Methods: Sc-PBGs with various calcium and sodium oxide contents were produced and characterised using thermal and spectroscopic analysis. Degradation behaviour, ion release, antibacterial action against Streptococcus mutans, anti-matrix metalloproteinase-2 (MMP-2) activity, remineralisation potential and in vivo biocompatibility were also investigated.
Results: The developed glass system showed linear Sc 3+ ions release over time. The released Sc 3+ shows statistically significant inhibition of S. mutans biofilm (1.2 log 10 CFU reduction at 6 h) and matrix metalloproteinase-2 (MMP-2) activity, compared with Sc-free glass and positive control. When Sc-PBGs were mounted alongside enamel sections, subjected to acidic challenges, alternating hyper- and hypomineralisation layers consistent with periods of re- and demineralisation were observed demonstrating their potential remineralising action. Furthermore, Sc-PBGs produced a non-toxic response when implanted subcutaneously for 2 weeks in Sprague Dawley rats.
Significance: Since Sc 3+ ions might act on various enzymes essential to the biological mechanisms underlying caries, Sc-PBGs could be a promising therapeutic agent against cariogenic bacteria.
(Copyright © 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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