Relevance of VEGF and CD147 in different SARS-CoV-2 positive digestive tracts characterized by thrombotic damage.

Autor: Bortolotti D; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Simioni C; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.; Laboratory for Technologies of Advanced Therapies (LTTA)-Electron Microscopy Center, University of Ferrara, Ferrara, Italy., Neri LM; Laboratory for Technologies of Advanced Therapies (LTTA)-Electron Microscopy Center, University of Ferrara, Ferrara, Italy.; Department of Translational Medicine, University of Ferrara, Ferrara, Italy., Rizzo R; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Semprini CM; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.; Medical Department, University Hospital of Ferrara Arcispedale Sant'Anna, Ferrara, Italy., Occhionorelli S; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.; Surgery Department, University Hospital of Ferrara Arcispedale Sant'Anna, Ferrara, Italy., Laface I; Department of Translational Medicine, University of Ferrara, Ferrara, Italy., Sanz JM; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Schiuma G; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Rizzo S; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Varano G; Department of Translational Medicine, University of Ferrara, Ferrara, Italy., Beltrami S; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Gentili V; Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy., Gafà R; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.; Oncological and Medical Department, University Hospital of Ferrara Arcispedale Sant'Anna, Ferrara, Italy., Passaro A; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.; Medical Department, University Hospital of Ferrara Arcispedale Sant'Anna, Ferrara, Italy.
Jazyk: angličtina
Zdroj: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2021 Dec; Vol. 35 (12), pp. e21969.
DOI: 10.1096/fj.202100821RRR
Abstrakt: Several evidence suggests that, in addition to the respiratory tract, also the gastrointestinal tract is a main site of severe acute respiratory syndrome CoronaVirus 2 (SARS-CoV-2) infection, as an example of a multi-organ vascular damage, likely associated with poor prognosis. To assess mechanisms SARS-CoV-2 responsible of tissue infection and vascular injury, correlating with thrombotic damage, specimens of the digestive tract positive for SARS-CoV-2 nucleocapsid protein were analyzed deriving from three patients, negative to naso-oro-pharyngeal swab for SARS-CoV-2. These COVID-19-negative patients came to clinical observation due to urgent abdominal surgery that removed different sections of the digestive tract after thrombotic events. Immunohistochemical for the expression of SARS-CoV-2 combined with a panel of SARS-CoV-2 related proteins angiotensin-converting enzyme 2 receptor, cluster of differentiation 147 (CD147), human leukocyte antigen-G (HLA-G), vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 was performed. Tissue samples were also evaluated by electron microscopy for ultrastructural virus localization and cell characterization. The damage of the tissue was assessed by ultrastructural analysis. It has been observed that CD147 expression levels correlate with SARS-CoV-2 infection extent, vascular damage and an increased expression of VEGF and thrombosis. The confirmation of CD147 co-localization with SARS-CoV-2 Spike protein binding on gastrointestinal tissues and the reduction of the infection level in intestinal epithelial cells after CD147 neutralization, suggest CD147 as a possible key factor for viral susceptibility of gastrointestinal tissue. The presence of SARS-CoV-2 infection of gastrointestinal tissue might be consequently implicated in abdominal thrombosis, where VEGF might mediate the vascular damage.
(© 2021 Federation of American Societies for Experimental Biology.)
Databáze: MEDLINE
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