On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells.

Autor:	Urazbaev A; National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan., Serikbaeva A; Laboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.; Department of Physiology and Biophysics (M/C 901), University of Illinois at Chicago, Chicago, IL, United States., Tvorogova A; A.N.Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Dusenbayev A; National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan., Kauanova S; Laboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.; Department of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, Kazakhstan., Vorobjev I; Laboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.; A.N.Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; Department of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, Kazakhstan.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2021 Nov 08; Vol. 8, pp. 745089. Date of Electronic Publication: 2021 Nov 08 (Print Publication: 2021).
DOI:	10.3389/fmolb.2021.745089
Abstrakt:	Microtubules are dynamic structures undergoing rapid growth and shrinkage in living cells and in vitro . The growth of microtubules in vitro was analyzed with subpixel precision (Maurer et al., Current Biology, 2014, 24 (4), 372-384); however, to what extent these results could be applied for microtubules growing in vivo remains largely unknown. Particularly, the question is whether microtubule growth velocity in cells could be sufficiently approximated by a Gaussian distribution or its variability requires a more sophisticated description? Addressing this question, we used time-lapse microscopy and mathematical modeling, and we analyzed EB-3 comets forming on microtubules of cultured cells with subpixel precision. Parameters of comets (shape, form, and velocity) were used as topological characteristics of 3D voxel objects. Using regression analysis, we determined the real positions of the microtubule tips in time-lapse sequences. By exponential decay fitting of the restored comet intensity profile, we found that in vivo EB-3 rapidly exchanges on growing microtubule ends with a decoration time ∼ 2 s. We next developed the model showing that the best correlation between comet length and microtubule end growth velocity is at time intervals close to the decoration time. In the cells, EB comet length positively correlates with microtubule growth velocity in preceding time intervals, while demonstrating no correlation in subsequent time intervals. Correlation between comet length and instantaneous growth velocity of microtubules remains under nocodazole treatment when mean values of both parameters decrease. Our data show that the growth of microtubules in living cells is well-approximated by a constant velocity with large stochastic fluctuations. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Urazbaev, Serikbaeva, Tvorogova, Dusenbayev, Kauanova and Vorobjev.)
Databáze:	MEDLINE
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