Seed-derived defensins from Scots pine: structural and functional features.

Autor: Shalovylo YI; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine., Yusypovych YM; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine., Hrunyk NI; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine., Roman II; Ivan Franko National University of Lviv, 1, Saksagansky St., Lviv, 79005, Ukraine., Zaika VK; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine., Krynytskyy HT; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine., Nesmelova IV; University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, 28223, USA., Kovaleva VA; Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine. kovaleva@nltu.edu.ua.
Jazyk: angličtina
Zdroj: Planta [Planta] 2021 Nov 24; Vol. 254 (6), pp. 129. Date of Electronic Publication: 2021 Nov 24.
DOI: 10.1007/s00425-021-03788-w
Abstrakt: Main Conclusion: The recombinant PsDef5.1 defensin inhibits the growth of phytopathogenic fungi, Gram-positive and Gram-negative bacteria, and human pathogen Candida albicans. Expression of seed-derived Scots pine defensins is tissue-specific and developmentally regulated. Plant defensins are ubiquitous antimicrobial peptides that possess a broad spectrum of activities and multi-functionality. The genes for these antimicrobial proteins form a multigenic family in the plant genome and are expressed in every organ. Most of the known defensins have been isolated from seeds of various monocot and dicot species, but seed-derived defensins have not yet been characterized in gymnosperms. This study presents the isolation of two new 249 bp cDNA sequences from Scots pine seeds with 97.9% nucleotide homology named PsDef5.1 and PsDef5.2. Their deduced amino acid sequences have typical plant defensin features, including an endoplasmic reticulum signal sequence of 31 amino acids (aa), followed by a characteristic defensin domain of 51 aa. To elucidate the functional activity of new defensins, we expressed the mature form of PsDef5.1 in a prokaryotic system. The purified recombinant peptide exhibited activity against the phytopathogenic fungi and Gram-negative and Gram-positive bacteria with the IC 50 of 5-18 µM. Moreover, it inhibited the growth of the human pathogen Candida albicans with the IC 50 of 6.0 µM. Expression analysis showed that transcripts of PsDef5.1-2 genes were present in immature and mature pine seeds and different parts of seedlings at the early stage of germination. In addition, unlike the PsDef5.2, the PsDef5.1 gene was expressed in the reproductive organs. Our findings indicate that novel defensins are promising candidates for transgenic application and the development of new antimicrobial drugs.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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