Kidney tissue engineering using a well-preserved acellular rat kidney scaffold and mesenchymal stem cells.
| Autor: | Shahraki S; Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Moghaddam Matin M; Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.; Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran., Ebrahimzadeh Bideskan A; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Aslzare M; Urology and Nephrology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Bahrami AR; Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.; Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran., Hosseinian S; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Iranpour S; Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran., Samadi Noshahr Z; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Khajavi Rad A; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Veterinary research forum : an international quarterly journal [Vet Res Forum] 2021; Vol. 12 (3), pp. 339-348. Date of Electronic Publication: 2021 Sep 15. |
| DOI: | 10.30466/vrf.2019.104640.2491 |
| Abstrakt: | The aim of this study was to acquire an effective method for preparation of rat decellularized kidney scaffolds capable of supporting proliferation and differentiation of human adipose tissue derived mesenchymal stem cells (AD-MSCs) into kidney cells. We compared two detergents, the sodium dodecyl sulfate (SDS) and triton X-100 for decellularization. The efficiency of these methods was assessed by Hematoxylin and Eosin (H&E), 4', 6 diamidino-2-phenylindole and immunohistochemistry (IHC) staining. In the next step, AD-MSCs were seeded into the SDS-treated scaffolds and assessed after three weeks of culture. Proliferation and differentiation of AD-MSCs into kidney-specific cell types were then analyzed by H&E and IHC staining. The histological examinations revealed that SDS was more efficient in removing kidney cells at all-time points compared to triton X-100. Also, in the SDS-treated sections the native extracellular matrix was more preserved than the triton-treated samples. Laminin was completely preserved during decellularization procedure using SDS. Cell attachment in the renal scaffold was observed after recellularization. Furthermore, differentiation of AD-MSCs into epithelial and endothelial cells was confirmed by expression of Na-K ATPase and vascular endothelial growth factor receptor 2 (VEGFR-2) in seeded rat renal scaffolds, respectively. Our findings illustrated that SDS was more effective for decellularization of rat kidney compared to triton X-100. We presented an optimized method for decellularization and recellularization of rat kidneys to create functional renal natural scaffolds. These natural scaffolds supported the growth of AD-MSCs and could also induce differentiation of these cells into epithelial and endothelial cells. Competing Interests: The authors have no financial conflicts of interest related to this study. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|