Potential fertilizer of spermatozoa in porcine epididymal tail post-orchiectomy.

Autor:	Ydiaquez-Miranda MA; Doctorate in Biological and Health Sciences, Metropolitan Autonomous University, ‎Mexico City, Mexico., Herrera-Barragán JA; Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, ‎ Mexico City, Mexico; ‎‎., González-Lozano M; Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, ‎ Mexico City, Mexico; ‎‎.; Center for Teaching, Research and Extension in Swine Production (CEIEPP), Faculty of ‎Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Jilotepec, Mexico., Ávalos-Rodríguez A; Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, ‎ Mexico City, Mexico; ‎‎.
Jazyk:	angličtina
Zdroj:	Veterinary research forum : an international quarterly journal [Vet Res Forum] 2021; Vol. 12 (3), pp. 267-272. Date of Electronic Publication: 2021 Sep 15.
DOI:	10.30466/vrf.2020.108342.2570
Abstrakt:	The aim of this study was to determine the potential fertilizing of spermatozoa from the epididymal tail in different periods of time post-orchiectomy (P-OQ). Therefore, the study was approached in two stages. In the first stage, the orchiectomy was performed in 30 adult pigs. The testicles were stored at 5.00 ˚C in physiological saline solution for 5, 24, 48, 72, 96 and 120 hr. The spermatozoa were obtained by backflushing the vas deferens. The spermogram and fluorometric study were performed for each sample to evaluate the exposure of phosphatidyl-serine (PS) and acrosome reaction (AR). The second stage included the fertilization test, 16 prepubertal sows were selected, after synchronizing the oestrous cycle and the post-cervical artificial insemination was performed with the refrigerated sperm samples from each P-OQ time. The percentage of live sperm remained without significant changes until 96 hr P-OQ. An increase in the percentage of spermatozoa that showed a PS exposure was observed. The premature AR was evident after 72 hr. Considering that the artificial insemination was performed ensuring a minimum number of live sperms, no significant differences were observed in the number of embryos and corpora lutea. The results indicated that pig sperm collected from the epididymal tail P-OQ and stored for 5 and up to 72 hr at 5.00 ˚C had viable characteristics and maintained their fertilization ability. However, there was an increase in the loss of phospholipid asymmetry of the plasma membrane as time increased (72 and 96 hr), therefore, sperm viability was decreased. Competing Interests: The authors declare that they have no competing interests.
Databáze:	MEDLINE
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