The microbial composition of the initial insult can predict the prognosis of experimental sepsis.

Autor: Tallósy SP; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Poles MZ; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Rutai A; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Fejes R; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Juhász L; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Burián K; Department of Medical Microbiology, Albert Szent-Györgyi Health Center and Faculty of Medicine, University of Szeged, Szeged, Hungary., Sóki J; Department of Medical Microbiology, Albert Szent-Györgyi Health Center and Faculty of Medicine, University of Szeged, Szeged, Hungary., Szabó A; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Boros M; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary., Kaszaki J; Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary. kaszaki.jozsef@med.u-szeged.hu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Nov 23; Vol. 11 (1), pp. 22772. Date of Electronic Publication: 2021 Nov 23.
DOI: 10.1038/s41598-021-02129-x
Abstrakt: We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague-Dawley rats. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Invasive monitoring was conducted in separate anesthetized groups (n = 12-13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. Death and ROFA scores peaked at 24 h. At this time, 20% mortality occurred in animals receiving a monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5; 5.0-7.0 and 5.0; 4.75-5.0, respectively). ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli cultures. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Initial and intraperitoneal microbiological testing should be used to improve translational research success.
(© 2021. The Author(s).)
Databáze: MEDLINE
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