Ultra-fast direct method for identifying microorganisms from BACTEC lytic/10 anaerobic/F flasks.

Autor: Urrutikoetxea-Gutiérrez M; Clinical Microbiology Department, Basurto University Hospital, 48013 Bilbao, Bizkaia, Spain.; Clinical Microbiology & Infection Control, ISS Biocruces Bizkaia, 48903 Cruces, Bizkaia, Spain., Sánchez Montiel M; Clinical Microbiology Department, Basurto University Hospital, 48013 Bilbao, Bizkaia, Spain., Vidal-Garcia M; Clinical Microbiology Department, Basurto University Hospital, 48013 Bilbao, Bizkaia, Spain.; Clinical Microbiology & Infection Control, ISS Biocruces Bizkaia, 48903 Cruces, Bizkaia, Spain., Basaras M; Inmunology, Microbiology & Parasitology Department, University of the Basque Country, 48940 Leioa, Bizkaia, Spain., Cisterna Cancer R; Inmunology, Microbiology & Parasitology Department, University of the Basque Country, 48940 Leioa, Bizkaia, Spain., Diaz de Tuesta Del Arco JL; Clinical Microbiology Department, Basurto University Hospital, 48013 Bilbao, Bizkaia, Spain.; Clinical Microbiology & Infection Control, ISS Biocruces Bizkaia, 48903 Cruces, Bizkaia, Spain.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2021 Dec; Vol. 16, pp. 1381-1387. Date of Electronic Publication: 2021 Nov 23.
DOI: 10.2217/fmb-2021-0059
Abstrakt: Background: Fast diagnosis of bloodstream infections remains the most important challenge for clinical microbiologists. The introduction of the mass-spectrometry represents a breakthrough, although several methods are already commonly used for the direct identification from positive blood cultures we present a faster method (ultra fast) for Lytic anaerobic flasks. Methods: We compare the ultra-fast (UF) method with the extensively employed differential centrifugation method (DC) and both to routine identification after 18-24 h of incubation. UF and DC method correlation rates to the gold standard were calculated, and statistical significance was proved with the Z test. Results: UF performed better overall than DC, with this difference being statistically significant. This tendency was observed in every subanalysis.
Databáze: MEDLINE
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