Phylogeography and phylogeny of Rhinoviruses collected from Severe Acute Respiratory Infection (SARI) cases over successive epidemic periods in Tunisia.

Autor:	Haddad-Boubaker S; Laboratory of Microbiology, Bechir Hamza Children's Hospital, Bab-Saadoun Square, Tunis, Tunisia., Ben Hamda C; Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia., Ghedira K; Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia., Mefteh K; Laboratory of Microbiology, Bechir Hamza Children's Hospital, Bab-Saadoun Square, Tunis, Tunisia.; Microbiology of Children and Immunocompromised, Faculty of Medicine of Tunis, University of Tunis El-Manar, Tunis, Tunisia., Bouafsoun A; Laboratory of Microbiology, Bechir Hamza Children's Hospital, Bab-Saadoun Square, Tunis, Tunisia.; Microbiology of Children and Immunocompromised, Faculty of Medicine of Tunis, University of Tunis El-Manar, Tunis, Tunisia., Boutiba-Ben Boubaker I; Laboratory of Microbiology, Charles Nicolle Hospital, Tunis, Tunisia.; Laboratory Research ''Antimicrobial Resistance', Faculty of Medicine of Tunis University of Tunis El-Manar, Tunis, Tunisia., Slim A; Laboratory of Microbiology, Charles Nicolle Hospital, Tunis, Tunisia., Menif K; Pediatric Intensive Care Unit, Bechir Hamza Children's Hospital in Tunis, Bab-Saadoun Square, Tunis Tunisia., Triki H; Laboratory of Microbiology, Bechir Hamza Children's Hospital, Bab-Saadoun Square, Tunis, Tunisia., Ben Hadj Kacem MA; Pediatric Intensive Care Unit, Bechir Hamza Children's Hospital in Tunis, Bab-Saadoun Square, Tunis Tunisia., Smaoui H; Laboratory of Microbiology, Bechir Hamza Children's Hospital, Bab-Saadoun Square, Tunis, Tunisia.; Microbiology of Children and Immunocompromised, Faculty of Medicine of Tunis, University of Tunis El-Manar, Tunis, Tunisia.
Jazyk:	angličtina
Zdroj:	PloS one [PLoS One] 2021 Nov 22; Vol. 16 (11), pp. e0259859. Date of Electronic Publication: 2021 Nov 22 (Print Publication: 2021).
DOI:	10.1371/journal.pone.0259859
Abstrakt:	Rhinoviruses (RV) are a major cause of Severe Acute Respiratory Infection (SARI) in children, with high genotypic diversity in different regions. However, RV type diversity remains unknown in several regions of the world. In this study, the genetic variability of the frequently circulating RV types in Northern Tunisia was investigated, using phylogenetic and phylogeographic analyses with a specific focus on the most frequent RV types: RV-A101 and RV-C45. This study concerned 13 RV types frequently circulating in Northern Tunisia. They were obtained from respiratory samples collected in 271 pediatric SARI cases, between September 2015 and November 2017. A total of 37 RV VP4-VP2 sequences, selected among a total of 49 generated sequences, was compared to 359 sequences from different regions of the world. Evolutionary analysis of RV-A101 and RV-C45 showed high genetic relationship between different Tunisian strains and Malaysian strains. RV-A101 and C45 progenitor viruses' dates were estimated in 1981 and 1995, respectively. Since the early 2000s, the two types had a wide spread throughout the world. Phylogenetic analyses of other frequently circulating strains showed significant homology of Tunisian strains from the same epidemic period, in contrast with earlier strains. The genetic relatedness of RV-A101 and RV-C45 might result from an introduction of viruses from different clades followed by local dissemination rather than a local persistence of an endemic clades along seasons. International traffic may play a key role in the spread of RV-A101, RV-C45, and other RVs. Competing Interests: The authors have declared that no competing interests exist.
Databáze:	MEDLINE
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