Identification of Polyphenolics from Loranthus globosus as Potential Inhibitors of Cholinesterase and Oxidative Stress for Alzheimer's Disease Treatment.

Autor: Kundo NK; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.; Department of Pharmacy, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh., Manik MIN; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.; Department of Pharmacy, Northern University, Dhaka 1205, Bangladesh., Biswas K; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.; Department of Pharmacy, East West University, Dhaka 1212, Bangladesh., Khatun R; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.; Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh., Al-Amin MY; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh., Alam AHMK; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh., Tanaka T; Department of Psychiatry, Osaka University Graduate School of Medicine, Osaka, Japan., Sadik G; Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2021 Nov 10; Vol. 2021, pp. 9154406. Date of Electronic Publication: 2021 Nov 10 (Print Publication: 2021).
DOI: 10.1155/2021/9154406
Abstrakt: Mistletoes are considered to be the potential medicinal herbs due to their rich traditional uses. Loranthus globosus is a Bangladeshi mango mistletoe that has been reported as folk medicine for various ailments and diseases. In an attempt to explore its effectiveness in Alzheimer's disease (AD), we investigated the antioxidant and acetylcholinesterase inhibitory activity of L . globosus . We report that the crude methanol extract (CME) of the plant contains a good amount of polyphenolics and possesses antioxidant and cholinesterase inhibitory activity. Fractionation of CME with solvents of varying polarity revealed the highest activity and polyphenolic content in the ethylacetate fraction (EAF). Correlation analysis revealed a significant ( P < 0.05) association of polyphenolics with the antioxidant and cholinesterase inhibitory properties. Using column chromatography with diaion resin, the polyphenolics (EAF-PP) were isolated from the EAF that displayed the potent antioxidant and cholinesterase inhibitory activities. Kinetic analysis showed that EAF-PP exhibited a competitive type of inhibition. A total of thirty-six compounds including catechin and its different derivatives were identified in the EAF-PP by LC/MS analysis. Bioactivity-guided separation approach afforded the isolation of the two major active compounds catechin and catechin dimer from the EAF-PP. Hence, EAF-PP represents a potential source of antioxidants and cholinesterase inhibitors, which can be used in the management of AD.
Competing Interests: The authors declare that there is no conflict of interests.
(Copyright © 2021 Netish Kumar Kundo et al.)
Databáze: MEDLINE
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