A novel hyperbaric swimming respirometer allows the simulation of varying swimming depths in fish respirometry studies.

Autor: Wysujack K; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany. Electronic address: klaus.wysujack@thuenen.de., Marohn L; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Lindemann C; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Illing B; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Freese M; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Pohlmann JD; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Reiser S; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Debes PV; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany; Department of Aquaculture and Fish Biology, Hólar University, 551 Sauðárkrókur, Iceland., Meskendahl L; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany., Pelster B; Institute of Zoology, University of Innsbruck, Innsbruck, Austria,; Center for Molecular Biosciences, University Innsbruck, Innsbruck, Austria., Hanel R; Thünen Institute of Fisheries Ecology, Herwigstraße 31, 27572 Bremerhaven, Germany.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2022 Feb; Vol. 264, pp. 111117. Date of Electronic Publication: 2021 Nov 15.
DOI: 10.1016/j.cbpa.2021.111117
Abstrakt: The understanding of swimming physiology and knowledge on the metabolic costs of swimming are important for assessing effects of environmental factors on migratory behavior. Swim tunnels are the most common experimental setups for measuring swimming performance and oxygen uptake rates in fishes; however, few can realistically simulate depth and the changes in hydrostatic pressure that many fishes experience, e.g. during diel vertical migrations. Here, we present a new hyperbaric swimming respirometer (HSR) that can simulate depths of up to 80 m. The system consists of three separate, identical swimming tunnels, each with a volume of 205 L, a control board and a storage tank with water treatment. The swimming chamber of each tunnel has a length of 1.40 m and a diameter of 20 cm. The HSR uses the principle of intermittent-flow respirometry and has here been tested with female European eels (Anguilla anguilla). Various pressure, temperature and flow velocity profiles can be programmed, and the effect on metabolic activity and oxygen consumption can be assessed. Thus, the HSR provides opportunities to study the physiology of fish during swimming in a simulated depth range that corresponds to many inland, coastal and shelf waters.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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