Pan-genome analysis identifies intersecting roles for Pseudomonas specialized metabolites in potato pathogen inhibition.

Autor:	Pacheco-Moreno A; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Stefanato FL; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Ford JJ; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Trippel C; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Uszkoreit S; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Ferrafiat L; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Grenga L; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Dickens R; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Kelly N; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Kingdon AD; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Ambrosetti L; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Nepogodiev SA; Department of Biochemistry and Metabolism, John Innes Centre, Norwich, United Kingdom., Findlay KC; Department of Cell and Developmental Biology, John Innes Centre, Norwich, United Kingdom., Cheema J; Department of Computational and Systems Biology, John Innes Centre, Norwich, United Kingdom., Trick M; Department of Computational and Systems Biology, John Innes Centre, Norwich, United Kingdom., Chandra G; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom., Tomalin G; VCS Potatoes, 2 Burnt Cottages, Framlingham, United Kingdom., Malone JG; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom.; School of Biological Sciences, University of East Anglia, Norwich, United Kingdom., Truman AW; Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Dec 31; Vol. 10. Date of Electronic Publication: 2021 Dec 31.
DOI:	10.7554/eLife.71900
Abstrakt:	Agricultural soil harbors a diverse microbiome that can form beneficial relationships with plants, including the inhibition of plant pathogens. Pseudomonas spp. are one of the most abundant bacterial genera in the soil and rhizosphere and play important roles in promoting plant health. However, the genetic determinants of this beneficial activity are only partially understood. Here, we genetically and phenotypically characterize the Pseudomonas fluorescens population in a commercial potato field, where we identify strong correlations between specialized metabolite biosynthesis and antagonism of the potato pathogens Streptomyces scabies and Phytophthora infestans . Genetic and chemical analyses identified hydrogen cyanide and cyclic lipopeptides as key specialized metabolites associated with S. scabies inhibition, which was supported by in planta biocontrol experiments. We show that a single potato field contains a hugely diverse and dynamic population of Pseudomonas bacteria, whose capacity to produce specialized metabolites is shaped both by plant colonization and defined environmental inputs. Competing Interests: AP, FS, JF, CT, LG, RD, NK, AK, LA, SN, KF, JC, MT, GC, JM, AT No competing interests declared, GT affiliated with VCS Potatoes and has no financial interests to declare (© 2021, Pacheco-Moreno et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu