Lippia graveolens HBK oleoresins, extracted by supercritical fluids, showed bactericidal activity against multidrug resistance Enterococcus faecalis and Staphylococcus aureus strains.

Autor: Calva-Cruz OJ; IPICYT, Instituto Potosino de Investigación Científica y Tecnológica A. C., San Luis Potosí, Mexico., Badillo-Larios NS; CICSaB, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autonoma de San Luis Potosí, San Luis Potosí, Mexico., De León-Rodríguez A; IPICYT, Instituto Potosino de Investigación Científica y Tecnológica A. C., San Luis Potosí, Mexico., Espitia-Rangel E; INIFAP, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Valle de México, Coatlinchán, Mexico., González-García R; Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico., Turrubiartes-Martinez EA; CICSaB, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autonoma de San Luis Potosí, San Luis Potosí, Mexico.; Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico., Castro-Gallardo A; Centro de Investigación para los Recursos Naturales, Antigua Normal Rural de Salaices, Chihuahua, México., Barba de la Rosa AP; IPICYT, Instituto Potosino de Investigación Científica y Tecnológica A. C., San Luis Potosí, Mexico.
Jazyk: angličtina
Zdroj: Drug development and industrial pharmacy [Drug Dev Ind Pharm] 2021 Oct; Vol. 47 (10), pp. 1546-1555. Date of Electronic Publication: 2022 Feb 20.
DOI: 10.1080/03639045.2021.2008417
Abstrakt: Objective: The aim of this work was to characterize Lippia graveolens oleoresins, obtained by Supercritical Fluid Extraction (SFE), from crops collected at different locations in Mexico. The antimicrobial effect of oleoresins was tested in reference strains and clinical isolates of susceptible and multidrug-resistant (MDR) strains of Enterococcus faecalis and Staphylococcus aureus .
Significance: The increasing of MDR strains is becoming a global public health problem that has led to the search for new treatments, and essential oils have resurged as a source of compounds with bactericidal functions. Oregano essential oil has attracted attention recently, however, this oil is mainly obtained by hydro-distillation (uses large amounts of water) or solvents extraction (potential contaminant). SFE has gained popularity as it represents an environmentally friendly technology.
Methods: L. graveolens oleoresins were obtained by SFE, total phenol contents were quantified by Folin-Ciocalteu method, the identification of compounds and thymol and carvacrol quantification was carried out by GC-MS. The antimicrobial activity was tested by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Results: SFE showed higher yields compared with the hydro-distillation process. L. graveolens grown in different Mexican locations showed differences in oleoresin composition and a slightly different antimicrobial capacity against clinical isolates.
Conclusions: It was demonstrated that SFE is an efficient technology for extracting L. graveolens oleoresins. Additionally, the solvent-free extraction method and the observed antimicrobial effect increase the applications of these oleoresins in fields, such as cosmetics, food industry, medicine, amongst others.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje