Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts.

Autor: Boriollo MFG; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil. marcelofgb@yahoo.com.br., Oliveira MC; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., Bassinello V; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., Aníbal PC; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., da Silva TA; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., da Silva JJ; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., Bassi RC; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., Netto MFR; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil., Dos Santos Dias CT; Department of Exact Sciences, College of Agriculture, University of São Paulo (ESALQ/USP), 11 Pádua Dias Ave, Piracicaba, SP, 13418-900, Brazil., Höfling JF; Department of Oral Diagnosis, Dental School of Piracicaba, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba, SP, 13414-903, Brazil.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2022 Mar; Vol. 26 (3), pp. 3061-3084. Date of Electronic Publication: 2021 Nov 18.
DOI: 10.1007/s00784-021-04290-z
Abstrakt: Objectives: This study evaluated the incidence of Candida species, and the genetic diversity and virulence of C. albicans of the oral cavity from patients with cleft lip and palate (CLP).
Materials and Methods: Oral samples were investigated by microbiological and species-specific PCR methods. The genetic diversity of C. albicans was established using isoenzyme markers, Nei's statistics, and clustering analysis. Hydrolytic enzymes (SAPs and PLs) were analyzed in vitro.
Results: Oral colonization by Candida species was observed in 29 patients with CLP (65.9%), and C. albicans was highly prevalent. SAP and PL activities were observed in 100% and 51.9% of isolates, respectively. High genetic diversity and patterns of monoclonal and polyclonal oral colonization by C. albicans were observed among patients with CLP. Two major polymorphic taxa (A and B) and other minor polymorphic taxa (C to J) were identified. Only one of the 16 clusters (taxon A) harbored strains from patients with and without CLP, whereas other clusters harbored strains exclusively from CLP patients.
Conclusions: The anatomical conditions of the oral cavity of patients with CLP contribute to the high incidence of Candida species (C. albicans, C. krusei, C. tropicalis, and/or Candida spp.). Data suggest high genetic diversity of potentially virulent C. albicans strains in the oral cavity of CLP patients.
Clinical Relevance: Microbiological niches in orofacial clefts can contribute to the emergence of a relative clinical genotypic identity of C. albicans. However, orofacial rehabilitation centers can contribute to the direct and indirect sources of transmission and propagation of Candida species.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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