Early Transcriptomic Response to Burn Injury: Severe Burns Are Associated With Immune Pathway Shutdown.
Autor: | Keyloun JW; Department of Surgery, MedStar Georgetown University Hospital, Washington, District of Columbia, USA.; Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, District of Columbia, USA., Campbell R; The Geneva Foundation, Silver Spring, Maryland, USA.; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Carney BC; Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, District of Columbia, USA.; Department of Surgery, Georgetown University School of Medicine, Washington, District of Columbia, USA.; Department of Biochemistry, Georgetown University School of Medicine, Washington, District of Columbia, USA., Yang R; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Miller SA; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Oak Ridge Institute for Science and Education, Silver Spring, Maryland, USA., Detwiler L; The Geneva Foundation, Silver Spring, Maryland, USA.; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Gautam A; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Moffatt LT; Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, District of Columbia, USA.; Department of Surgery, Georgetown University School of Medicine, Washington, District of Columbia, USA.; Department of Biochemistry, Georgetown University School of Medicine, Washington, District of Columbia, USA., Hammamieh R; Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Jett M; Headquarters Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Shupp JW; Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, District of Columbia, USA.; Department of Surgery, Georgetown University School of Medicine, Washington, District of Columbia, USA.; Department of Biochemistry, Georgetown University School of Medicine, Washington, District of Columbia, USA. |
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Jazyk: | angličtina |
Zdroj: | Journal of burn care & research : official publication of the American Burn Association [J Burn Care Res] 2022 Mar 23; Vol. 43 (2), pp. 306-314. |
DOI: | 10.1093/jbcr/irab217 |
Abstrakt: | Burn injury induces a systemic hyperinflammatory response with detrimental side effects. Studies have described the biochemical changes induced by severe burns, but the transcriptome response is not well characterized. The goal of this work is to characterize the blood transcriptome after burn injury. Burn patients presenting to a regional center between 2012 and 2017 were prospectively enrolled. Blood was collected on admission and at predetermined time points (hours 2, 4, 8, 12, and 24). RNA was isolated and transcript levels were measured with a gene expression microarray. To identify differentially regulated genes (false-discovery rate ≤0.1) by burn injury severity, patients were grouped by TBSA above or below 20% and statistically enriched pathways were identified. Sixty-eight patients were analyzed, most patients were male with a median age of 41 (interquartile range, 30.5-58.5) years, and TBSA of 20% (11%-34%). Thirty-five patients had % TBSA injury ≥20%, and this group experienced greater mortality (26% vs 3%, P = .008). Comparative analysis of genes from patients with ≥20% TBSA revealed 1505, 613, 380, 63, 1357, and 954 differentially expressed genes at hours 0, 2, 4, 8, 12, and 24, respectively. Pathway analysis revealed an initial up-regulation in several immune/inflammatory pathways within the ≥20% TBSA groups followed by shutdown. Severe burn injury is associated with an early proinflammatory immune response followed by shutdown of these pathways. Examination of the immunoinflammatory response to burn injury through differential gene regulation and associated immune pathways by injury severity may identify mechanistic targets for future intervention. (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Burn Association. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
Databáze: | MEDLINE |
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