A procedure for removal of cyanuric acid in swimming pools using a cell-free thermostable cyanuric acid hydrolase.

Autor: Guo F; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., McAuliffe JC; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Bongiorni C; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Latone JA; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Pepsin MJ; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Chow MS; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Dhaliwal RS; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Hoffmann KM; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Brazil BT; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Heng MH; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA., Robinson SL; Department of Environmental Microbiology, Eawag: Swiss Federal Institute for Aquatic Science and Technology, Überlandstrasse 133, CH-8600 Dübendorf, Switzerland., Wackett LP; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, 55455 MN, USA., Whited GM; Nutrition & Biosciences, International Flavors and Fragrances Inc., Palo Alto, 94304 CA, USA.
Jazyk: angličtina
Zdroj: Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2022 Apr 14; Vol. 49 (2).
DOI: 10.1093/jimb/kuab084
Abstrakt: Cyanuric acid (CYA) is used commercially for maintaining active chlorine to inactivate microbial and viral pathogens in swimming pools and hot tubs. Repeated CYA addition can cause a lack of available chlorine and adequate disinfection. Acceptable CYA levels can potentially be restored via cyanuric acid hydrolases (CAH), enzymes that hydrolyze CYA to biuret under mild conditions. Here we describe a previously unknown CAH enzyme from Pseudolabrys sp. Root1462 (CAH-PR), mined from public databases by bioinformatic analysis of potential CAH genes, which we show to be suitable in a cell-free form for industrial applications based upon favorable enzymatic and physical properties, combined with high-yield expression in aerobic cell culture. The kinetic parameters and modeled structure were similar to known CAH enzymes, but the new enzyme displayed a surprising thermal and storage stability. The new CAH enzyme was applied, following addition of inexpensive sodium sulfite, to hydrolyze CYA to biuret. At the desired endpoint, hypochlorite addition inactivated remaining enzyme and oxidized biuret to primarily dinitrogen and carbon dioxide gases. The mechanism of biuret oxidation with hypochlorite under conditions relevant to recreational pools is described.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.)
Databáze: MEDLINE
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