From apolar gastrula to polarized larva: Embryonic development of a marine hydroid, Dynamena pumila.

Autor: Vetrova AA; Laboratory of Morphogenesis Evolution, Koltzov Institute of Developmental Biology RAS, Moscow, Russia., Lebedeva TS; Department of Neurosciences and Developmental Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria., Saidova AA; Department of Cell Biology and Histology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia., Kupaeva DM; Laboratory of Morphogenesis Evolution, Koltzov Institute of Developmental Biology RAS, Moscow, Russia., Kraus YA; Laboratory of Morphogenesis Evolution, Koltzov Institute of Developmental Biology RAS, Moscow, Russia.; Department of Evolutionary Biology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia., Kremnyov SV; Laboratory of Morphogenesis Evolution, Koltzov Institute of Developmental Biology RAS, Moscow, Russia.; Department of Embryology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2022 May; Vol. 251 (5), pp. 795-825. Date of Electronic Publication: 2021 Dec 01.
DOI: 10.1002/dvdy.439
Abstrakt: Background: In almost all metazoans examined to this respect, the axial patterning system based on canonical Wnt (cWnt) signaling operates throughout the course of development. In most metazoans, gastrulation is polar, and embryos develop morphological landmarks of axial polarity, such as blastopore under control/regulation from cWnt signaling. However, in many cnidarian species, gastrulation is morphologically apolar. The question remains whether сWnt signaling providing the establishment of a body axis controls morphogenetic processes involved in apolar gastrulation.
Results: In this study, we focused on the embryonic development of Dynamena pumila, a cnidarian species with apolar gastrulation. We thoroughly described cell behavior, proliferation, and ultrastructure and examined axial patterning in the embryos of this species. We revealed that the first signs of morphological polarity appear only after the end of gastrulation, while molecular prepatterning of the embryo does exist during gastrulation. We have shown experimentally that in D. pumila, the direction of the oral-aboral axis is highly robust against perturbations in cWnt activity.
Conclusions: Our results suggest that morphogenetic processes are uncoupled from molecular axial patterning during gastrulation in D. pumila. Investigation of D. pumila might significantly expand our understanding of the ways in which morphological polarization and axial molecular patterning are linked in Metazoa.
(© 2021 American Association for Anatomy.)
Databáze: MEDLINE
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