Using recombinant adhesive proteins as durable and green flame-retardant coatings.

Autor:	Leong WI; Pui Ching Middle School (Macau), Edificio Pui Ching, 7A Av. de Horta e Costa, Macau SAR, China., Lo OLI; Pui Ching Middle School (Macau), Edificio Pui Ching, 7A Av. de Horta e Costa, Macau SAR, China.; The Centre for Inflammation Research, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, United Kingdom., Cheng FT; Pui Ching Middle School (Macau), Edificio Pui Ching, 7A Av. de Horta e Costa, Macau SAR, China., Cheong WM; Pui Ching Middle School (Macau), Edificio Pui Ching, 7A Av. de Horta e Costa, Macau SAR, China., Seak LCU; Pui Ching Middle School (Macau), Edificio Pui Ching, 7A Av. de Horta e Costa, Macau SAR, China.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 3DY, United Kingdom.
Jazyk:	angličtina
Zdroj:	Synthetic and systems biotechnology [Synth Syst Biotechnol] 2021 Nov 05; Vol. 6 (4), pp. 369-376. Date of Electronic Publication: 2021 Nov 05 (Print Publication: 2021).
DOI:	10.1016/j.synbio.2021.10.005
Abstrakt:	Current fire retardants are known to be toxic to humans and our environment. As environmental-friendly flame retardants (FRs), protein-based flame retardants have been studied extensively recently, even though they are not durable. In this study, we designed, synthesized and tested a durable protein-based FR through the fusion of the adhesion domain from either mussel foot protein-5 (mfp-5) or cellulose-binding domain (CBD) with flame retardant protein (SR protein and alpha casein). We first verified the expression of the recombinant proteins in Escherichia coli using Western blot. Then, we coated the fusion protein (carrying cell lysates) to cotton fabrics and wood and verified with Infrared (IR) spectroscopy. Using a vertical burning test and wood flammability test, we confirmed the flame retardancy of the materials after the protein coating. In the vertical burning test, the SR protein and alpha casein flame retardant proteins with the CBD adhesion domain showed a 50.0% and 43.3% increase in flame retardancy. The data is also consistent in the wood flame retardancy test. Confocal imaging experiments also suggested these new fire retardants can be preserved on the materials well even after washing. Overall, our results showed that flame-retardant proteins with adhesion domains are high potential candidates of green alternative flame retardants. Competing Interests: We have no conflict of interest to declare. This statement is to certify that all Authors have seen and approved the manuscript being submitted. We warrant that the article is the Authors' original work. We warrant that the article has not received prior publication and is not under consideration for publication elsewhere. On behalf of all Co-Authors, the corresponding Author shall bear full responsibility for the submission. This research has not been submitted for publication nor has it been published in whole or in part elsewhere. We attest to the fact that all Authors listed on the title page have contributed significantly to the work, have read the manuscript, attest to the validity and legitimacy of the data and its interpretation, and agree to its submission to the Synthetic and Systems Biotechnology. All authors agree that author list is correct in its content and order and that no modification to the author list can be made without the formal approval of the Editor-in-Chief, and all authors accept that the Editor-in-Chief's decisions over acceptance or rejection or in the event of any breach of the Principles of Ethical Publishing in the Synthetic and Systems Biotechnology being discovered of retraction are final. No additional authors will be added post submission, unless editors receive agreement from all authors and detailed information is supplied as to why the author list should be amended. (© 2021 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu