Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution.

Autor: Williams GOS; Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK., Williams E; Centre for Advanced Instrumentation, Department of Physics, Durham University, South Road, Durham, DH1 3LE, UK., Finlayson N; School of Engineering, Institute for Integrated Micro and Nano Systems, University of Edinburgh, King's Buildings, Alexander Crum Brown Road, Edinburgh, EH9 3FF, UK., Erdogan AT; School of Engineering, Institute for Integrated Micro and Nano Systems, University of Edinburgh, King's Buildings, Alexander Crum Brown Road, Edinburgh, EH9 3FF, UK., Wang Q; Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK., Fernandes S; Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK., Akram AR; Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK., Dhaliwal K; Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK., Henderson RK; School of Engineering, Institute for Integrated Micro and Nano Systems, University of Edinburgh, King's Buildings, Alexander Crum Brown Road, Edinburgh, EH9 3FF, UK., Girkin JM; Centre for Advanced Instrumentation, Department of Physics, Durham University, South Road, Durham, DH1 3LE, UK. j.m.girkin@durham.ac.uk., Bradley M; School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, UK. Mark.Bradley@ed.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Nov 16; Vol. 12 (1), pp. 6616. Date of Electronic Publication: 2021 Nov 16.
DOI: 10.1038/s41467-021-26837-0
Abstrakt: The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems.
(© 2021. The Author(s).)
Databáze: MEDLINE
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