The different activities of RNA G-quadruplex structures are controlled by flanking sequences.

Autor:	Zheng AJ; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France., Thermou A; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France.; ICCVS, University of Gdańsk, Science, Gdańsk, Poland., Guixens Gallardo P; CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, Orsay, France.; CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, Orsay, France., Malbert-Colas L; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France., Daskalogianni C; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France.; ICCVS, University of Gdańsk, Science, Gdańsk, Poland., Vaudiau N; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France., Brohagen P; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France., Granzhan A; CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, Orsay, France.; CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, Orsay, France., Blondel M; Inserm UMR1078, Université de Bretagne Occidentale (UBO), Etablissement Français du Sang (EFS) Bretagne, CHRU Brest, Brest, France., Teulade-Fichou MP; CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, Orsay, France.; CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, Orsay, France., Martins RP; ISP, INRAE, Université de Tours, UMR1282, Tours, France., Fahraeus R; Inserm UMRS1131, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, Paris, France robin.fahraeus@inserm.fr.; RECAMO, Masaryk Memorial Cancer Institute, Brno, Czech Republic.; Department of Medical Biosciences, Umeå University, Umeå, Sweden.; ICCVS, University of Gdańsk, Science, Gdańsk, Poland.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2021 Nov 16; Vol. 5 (2). Date of Electronic Publication: 2021 Nov 16 (Print Publication: 2022).
DOI:	10.26508/lsa.202101232
Abstrakt:	The role of G-quadruplex (G4) RNA structures is multifaceted and controversial. Here, we have used as a model the EBV-encoded EBNA1 and the Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded LANA1 mRNAs. We have compared the G4s in these two messages in terms of nucleolin binding, nuclear mRNA retention, and mRNA translation inhibition and their effects on immune evasion. The G4s in the EBNA1 message are clustered in one repeat sequence and the G4 ligand PhenDH2 prevents all G4-associated activities. The RNA G4s in the LANA1 message take part in similar multiple mRNA functions but are spread throughout the message. The different G4 activities depend on flanking coding and non-coding sequences and, interestingly, can be separated individually. Together, the results illustrate the multifunctional, dynamic and context-dependent nature of G4 RNAs and highlight the possibility to develop ligands targeting specific RNA G4 functions. The data also suggest a common multifunctional repertoire of viral G4 RNA activities for immune evasion. (© 2021 Zheng et al.)
Databáze:	MEDLINE
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