Rapid qualitative profiling and quantitative analysis of Juglandis Mandshuricae Cortex and seven flavonoids by ultra-high performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry.

Autor: Sun Z; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Zhao M; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Zuo L; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Zhou S; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, P. R. China., Fan F; Department of Neurointerventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Jia Q; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Xue L; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Li H; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, P. R. China., Kang J; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China., Zhang X; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2022 Jan; Vol. 45 (2), pp. 518-528. Date of Electronic Publication: 2021 Nov 25.
DOI: 10.1002/jssc.202100658
Abstrakt: Juglandis Mandshuricae Cortex is the bark of Juglans mandshurica Maxim., which has been used as a folk medicine plant in China and India. In this study, an ultra-high performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry method was developed to clarify and quantify the chemical profiling of Juglandis Mandshuricae Cortex rapidly. A total of 113 compounds were characterized. Among them, seven flavonoids were simultaneously quantified in 15 min, including myricetin, myricetrin, taxifolin, kaempferol, quercetin, quercitrin, and naringenin. The method was validated for accuracy, precision, and the limits of detection and quantification. All calibration curves showed a good linear relationship (r > 0.9990) within test ranges. The intra- and inter-day relative standard deviations were less than 2.16%. Accuracy validation showed that the recovery was between 95.6 and 101.3% with relative standard deviation values below 2.85%. The validated method was successfully applied to determine the contents of seven flavones in Juglandis Mandshuricae Cortex from seven sources and the contents of these places were calculated respectively. This method provides a theoretical basis for further developing the medicinal value of Juglandis Mandshuricae Cortex.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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