Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9.
Autor: | Assou J; Institute of Horticultural Production Systems, Leibniz Universität Hannover, Hannover, Germany., Zhang D; Department of Plant Biotechnology, Institute of Plant Genetics, Leibniz Universität Hannover, Hannover, Germany., Roth KDR; Department of Biotechnology, Institute of Biochemistry, Biotechnology, and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany., Steinke S; Department of Biotechnology, Institute of Biochemistry, Biotechnology, and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany., Hust M; Department of Biotechnology, Institute of Biochemistry, Biotechnology, and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany., Reinard T; Department of Plant Biotechnology, Institute of Plant Genetics, Leibniz Universität Hannover, Hannover, Germany., Winkelmann T; Institute of Horticultural Production Systems, Leibniz Universität Hannover, Hannover, Germany., Boch J; Department of Plant Biotechnology, Institute of Plant Genetics, Leibniz Universität Hannover, Hannover, Germany. |
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Jazyk: | angličtina |
Zdroj: | The Plant journal : for cell and molecular biology [Plant J] 2022 Feb; Vol. 109 (3), pp. 649-663. Date of Electronic Publication: 2021 Dec 02. |
DOI: | 10.1111/tpj.15584 |
Abstrakt: | Food allergies are a major health issue worldwide. Modern breeding techniques such as genome editing via CRISPR/Cas9 have the potential to mitigate this by targeting allergens in plants. This study addressed the major allergen Bra j I, a seed storage protein of the 2S albumin class, in the allotetraploid brown mustard (Brassica juncea). Cotyledon explants of an Indian gene bank accession (CR2664) and the German variety Terratop were transformed using Agrobacterium tumefaciens harboring binary vectors with multiple single guide RNAs to induce either large deletions or frameshift mutations in both Bra j I homoeologs. A total of 49 T (© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.) |
Databáze: | MEDLINE |
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