Encapsulation of the psyllid-pathogenic fungus Pandora sp. nov. inedit. and experimental infection of target insects.

Autor: Muskat LC; Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany.; Agricultural Entomology, Department of Crop Sciences, Georg-August-University Goettingen, Goettingen, Germany., Görg LM; Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Institute for Cultivated Plants, Dossenheim, Germany., Humbert P; Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany., Gross J; Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Institute for Cultivated Plants, Dossenheim, Germany., Eilenberg J; Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark., Patel AV; Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2022 Mar; Vol. 78 (3), pp. 991-999. Date of Electronic Publication: 2021 Dec 03.
DOI: 10.1002/ps.6710
Abstrakt: Background: Pandora sp. nov. inedit. (Entomophthorales: Entomophthoraceae) (ARSEF 13372) is a recently isolated entomophthoralean fungus with potential for psyllid pest control. This study aimed to develop a formulation based on biocompatible hydrogel beads in order to transfer the fungus into an easily applicable form and to test the effects on psyllids.
Results: After encapsulation in calcium alginate beads, Pandora sp. nov. grew from the beads and discharged conidia over 12 days under optimal humidity conditions at 18 °C. Conidial number was increased 2.95-fold by the addition of skimmed milk as nutritional formulation adjuvant to the beads. The virulence of the encapsulated fungus was assessed with the two target psyllid species; the summer apple psyllid, Cacopsylla picta and the pear psyllid, Cacopsylla pyri. Beads containing skimmed milk as nutritional adjuvant led to the highest mortalities (48.3% on C. picta and 75.0% on C. pyri). In a second bioassay, survival time of C. pyri exposed to beads containing different concentration (10%, 20% or 40%) of Pandora sp. nov. was tested. The survival time of C. pyri was significantly reduced when exposed to beads containing 10% or 20% Pandora sp. nov. The median survival time was reached after 5-6 days past inoculation and the cumulative mortality for C. pyri treated with Pandora sp. nov. beads showed up to 89% mortality.
Conclusion: The promising results of this study will ease the way for large-scale field application of a novel Pandora species in biological psyllid pest control.
(© 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
Databáze: MEDLINE
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