SARS-CoV-2 monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data.

Autor: Nagarkar M; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Nagarkar.Maitreyi@epa.gov., Keely SP; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Keely.Scott@epa.gov., Jahne M; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Jahne.Michael@epa.gov., Wheaton E; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Wheaton.Emily@epa.gov., Hart C; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Hart.Chloe@epa.gov., Smith B; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Smith.Bruce@epa.gov., Garland J; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Garland.Jay@epa.gov., Varughese EA; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Varughese.Eunice@epa.gov., Braam A; APTIM Corp., 4171 Essen Lane, Baton Rouge, LA 70809. Electronic address: Braam.Ana@epa.gov., Wiechman B; APTIM Corp., 4171 Essen Lane, Baton Rouge, LA 70809. Electronic address: Wiechman.Barry@epa.gov., Morris B; Pegasus Technical Services Inc., 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Morris.Brian@epa.gov., Brinkman NE; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Brinkman.Nichole@epa.gov.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Apr 10; Vol. 816, pp. 151534. Date of Electronic Publication: 2021 Nov 13.
DOI: 10.1016/j.scitotenv.2021.151534
Abstrakt: Wastewater monitoring of SARS-CoV-2 presents a means of tracking COVID-19 community infection dynamics on a broader geographic scale. However, accounting for environmental and sample-processing losses may be necessary for wastewater measurements to readily inform our understanding of infection prevalence. Here, we present measurements of the SARS-CoV-2 N1 and N2 gene targets from weekly wastewater samples at three sites in Hamilton County, Ohio, during an increase and subsequent decline of COVID-19 infections. The concentration of N1 or N2 RNA in wastewater, measured over the course of six months, ranged from below the detection limit to over 10 4 gene copies/l, and correlated with case data at two wastewater treatment plants, but not at a sub-sewershed-level sampling site. We also evaluated the utility of a broader range of variables than has been reported consistently in previous work, in improving correlations of SARS-CoV-2 concentrations with case data. These include a spiked matrix recovery control (OC43), flow-normalization, and assessment of fecal loading using endogenous fecal markers (HF183, PMMoV, crAssphage). We found that adjusting for recovery, flow, and fecal indicators increased these correlations for samples from a larger sewershed (serving ~488,000 people) with greater industrial and stormwater inputs, but raw N1/N2 concentrations corresponded better with case data at a smaller, residential-oriented sewershed. Our results indicate that the optimal adjustment factors for correlating wastewater and clinical case data moving forward may not be generalizable to all sewersheds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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