Evaluation of a bioaerosol sampler for indoor environmental surveillance of Severe Acute Respiratory Syndrome Coronavirus 2.

Autor: Horve PF; Biology and the Built Environment Center, University of Oregon, Eugene, OR, United States of America., Dietz L; Biology and the Built Environment Center, University of Oregon, Eugene, OR, United States of America., Northcutt D; Biology and the Built Environment Center, University of Oregon, Eugene, OR, United States of America.; Energy Studies in Buildings Laboratory, University of Oregon, Eugene, OR, United States of America., Stenson J; Biology and the Built Environment Center, University of Oregon, Eugene, OR, United States of America.; Energy Studies in Buildings Laboratory, University of Oregon, Eugene, OR, United States of America., Van Den Wymelenberg K; Biology and the Built Environment Center, University of Oregon, Eugene, OR, United States of America.; Energy Studies in Buildings Laboratory, University of Oregon, Eugene, OR, United States of America.; Institute for Health and the Built Environment, University of Oregon, Portland, OR, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Nov 15; Vol. 16 (11), pp. e0257689. Date of Electronic Publication: 2021 Nov 15 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0257689
Abstrakt: The worldwide spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has ubiquitously impacted many aspects of life. As vaccines continue to be manufactured and administered, limiting the spread of SARS-CoV-2 will rely more heavily on the early identification of contagious individuals occupying reopened and increasingly populated indoor environments. In this study, we investigated the utility of an impaction-based bioaerosol sampling system with multiple nucleic acid collection media. Heat-inactivated SARS-CoV-2 was utilized to perform bench-scale, short-range aerosol, and room-scale aerosol experiments. Through bench-scale experiments, AerosolSense Capture Media (ACM) and nylon flocked swabs were identified as the highest utility media. In room-scale aerosol experiments, consistent detection of aerosol SARS-CoV-2 was achieved at an estimated aerosol concentration equal to or greater than 0.089 genome copies per liter of room air (gc/L) when air was sampled for eight hours or more at less than one air change per hour (ACH). Shorter sampling periods (75 minutes) yielded consistent detection at ~31.8 gc/L of room air and intermittent detection down to ~0.318 gc/L at (at both 1 and 6 ACH). These results support further exploration in real-world testing scenarios and suggest the utility of indoor aerosol surveillance as an effective risk mitigation strategy in occupied buildings.
Competing Interests: Van Den Wymelenberg has a company called Duktile through which he provides healthy building consulting, including consulting related to viral pathogens, and he serves as a scientific advisor to EnviralTech, a company that conducts viral surface environmental surveillance, including in senior care facilities. This does not alter our adherence to PLOS ONE policies on sharing data and materials
Databáze: MEDLINE
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