Determination of VP2 sequence-based virulence motifs and phylogenetic analysis of domestic Turkish IPNV ısolates.

Autor: Tamer C; Faculty of Veterinary Medicine, Department of Virology, Ondokuz Mayıs University, Samsun, Turkey., Isıdan H; Faculty of Veterinary Medicine, Department of Virology, Sivas Cumhuriyet University, Sivas, Turkey., Kalaycı G; Republic of Turkey Ministry Of Agriculture And Forestry, Izmir Bornova Veterinary Control Institute, Izmir, Turkey., Ozan E; Faculty of Veterinary Medicine, Department of Veterinary Experimental Animals, Ondokuz Mayıs University, Samsun, Turkey., Ozkan B; Republic of Turkey Ministry Of Agriculture And Forestry, Izmir Bornova Veterinary Control Institute, Izmir, Turkey., Albayrak H; Faculty of Veterinary Medicine, Department of Virology, Ondokuz Mayıs University, Samsun, Turkey.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2022 Feb; Vol. 45 (2), pp. 327-334. Date of Electronic Publication: 2021 Nov 14.
DOI: 10.1111/jfd.13558
Abstrakt: Infectious pancreatic necrosis (IPN) is a highly contagious disease of young salmonid fish and is one of the most severe economic diseases in aquaculture. In Turkey, an increase in infectious pancreatic necrosis virus (IPNV) outbreaks in freshwater rainbow trout have been reported in recent years. This study aimed to analyze the VP2 gene from recent IPNV isolates from Turkey to determine whether there are epidemiological links between IPNV isolates from rainbow trout (Oncorhynchus mykiss; 62) and sea bass (Dicentrarchus labrax; 1), wild turbot (Scophthalmus maximus; 1) and the environment in order to investigate potential wild and farmed fish interactions. In this study, 62 Turkish IPNV isolates collected over 10 years (2005-2014) from rainbow trout, sea bass and turbot were genotypically characterized. The phylogenetic analysis indicated that Turkish IPNV isolates are closely related to strains from Denmark, Iran and Spain and that all Turkish IPNV isolates belong to genogroup V, serotype A2 (Sp strain). Furthermore, low genetic diversity was found among the Turkish isolates (identity, 95.5%-100% nucleotides and 97.8%-100% amino acids). The result of the analysis of the amino acid residues found at positions 217, 221 and 247 (proline, threonine and alanine, respectively) could be associated with virulence.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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