The Host Response to Viral Infections Reveals Common and Virus-Specific Signatures in the Peripheral Blood.

Autor:	Tsalik EL; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Emergency Department Service, Durham Veterans Affairs Health Care System, Durham, NC, United States.; Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States., Fiorino C; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States., Aqeel A; Duke Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, United States., Liu Y; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States., Henao R; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Department of Electrical and Computer Engineering, Duke University, Durham, NC, United States., Ko ER; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Department of Medicine, Duke Regional Hospital, Durham, NC, United States., Burke TW; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States., Reller ME; Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States., Bodinayake CK; Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka., Nagahawatte A; Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka., Arachchi WK; Teaching Hospital Karapitiya, Galle, Sri Lanka., Devasiri V; Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka., Kurukulasooriya R; Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka., McClain MT; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States.; Medical Service, Durham Veterans Affairs Health Care System, Durham, NC, United States., Woods CW; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States.; Medical Service, Durham Veterans Affairs Health Care System, Durham, NC, United States., Ginsburg GS; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States., Tillekeratne LG; Duke Center for Applied Genomics and Precision Medicine, Duke University School of Medicine, Durham, NC, United States.; Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States.; Medical Service, Durham Veterans Affairs Health Care System, Durham, NC, United States., Schughart K; Department of Infection Genetics, Helmholtz Centre for Infection Research, Braunschweig, Germany.; University of Veterinary Medicine Hannover, Hannover, Germany.; Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2021 Oct 27; Vol. 12, pp. 741837. Date of Electronic Publication: 2021 Oct 27 (Print Publication: 2021).
DOI:	10.3389/fimmu.2021.741837
Abstrakt:	Viruses cause a wide spectrum of clinical disease, the majority being acute respiratory infections (ARI). In most cases, ARI symptoms are similar for different viruses although severity can be variable. The objective of this study was to understand the shared and unique elements of the host transcriptional response to different viral pathogens. We identified 162 subjects in the US and Sri Lanka with infections due to influenza, enterovirus/rhinovirus, human metapneumovirus, dengue virus, cytomegalovirus, Epstein Barr Virus, or adenovirus. Our dataset allowed us to identify common pathways at the molecular level as well as virus-specific differences in the host immune response. Conserved elements of the host response to these viral infections highlighted the importance of interferon pathway activation. However, the magnitude of the responses varied between pathogens. We also identified virus-specific responses to influenza, enterovirus/rhinovirus, and dengue infections. Influenza-specific differentially expressed genes (DEG) revealed up-regulation of pathways related to viral defense and down-regulation of pathways related to T cell and neutrophil responses. Functional analysis of entero/rhinovirus-specific DEGs revealed up-regulation of pathways for neutrophil activation, negative regulation of immune response, and p38MAPK cascade and down-regulation of virus defenses and complement activation. Functional analysis of dengue-specific up-regulated DEGs showed enrichment of pathways for DNA replication and cell division whereas down-regulated DEGs were mainly associated with erythrocyte and myeloid cell homeostasis, reactive oxygen and peroxide metabolic processes. In conclusion, our study will contribute to a better understanding of molecular mechanisms to viral infections in humans and the identification of biomarkers to distinguish different types of viral infections. Competing Interests: ELT, GSG, CWW, and TWB consult for and hold equity in Biomeme, Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Tsalik, Fiorino, Aqeel, Liu, Henao, Ko, Burke, Reller, Bodinayake, Nagahawatte, Arachchi, Devasiri, Kurukulasooriya, McClain, Woods, Ginsburg, Tillekeratne and Schughart.)
Databáze:	MEDLINE
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