Moderate Physical Exercise Activates ATR 2 Receptors, Improving Inflammation and Oxidative Stress in the Duodenum of 2K1C Hypertensive Rats.

Autor: da Silva ACA; Graduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil.; Laboratory of Exercise and Gastrointestinal Tract, Department of Physical Education, Federal University of Piauí, Teresina, Brazil., Severo JS; Laboratory of Exercise and Gastrointestinal Tract, Department of Physical Education, Federal University of Piauí, Teresina, Brazil.; Graduate Program in Food and Nutrition, Federal University of Piauí, Teresina, Brazil., Dos Santos BLB; Laboratory of Exercise and Gastrointestinal Tract, Department of Physical Education, Federal University of Piauí, Teresina, Brazil., Mendes PHM; Laboratory of Exercise and Gastrointestinal Tract, Department of Physical Education, Federal University of Piauí, Teresina, Brazil., Nobre LMS; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., de Oliveira AP; Graduate Program in Biotechnology, Federal University of Delta do Parnaíba, Parnaíba, Brazil., Ferreira FCS; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Medeiros JVR; Graduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil.; Graduate Program in Biotechnology, Federal University of Delta do Parnaíba, Parnaíba, Brazil., Lima-Junior RC; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Havt A; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Palheta-Junior RC; School of Veterinary Medicine, Federal University of Vale do São Francisco, Petrolina, Brazil., Dos Santos AA; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Tolentino M; Graduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil.; Laboratory of Exercise and Gastrointestinal Tract, Department of Physical Education, Federal University of Piauí, Teresina, Brazil.; Graduate Program in Food and Nutrition, Federal University of Piauí, Teresina, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in physiology [Front Physiol] 2021 Oct 14; Vol. 12, pp. 734038. Date of Electronic Publication: 2021 Oct 14 (Print Publication: 2021).
DOI: 10.3389/fphys.2021.734038
Abstrakt: Background: In addition to the cardiovascular and renal systems, the gastrointestinal tract also contains angiotensin ATR 1a , ATR 1b , and ATR 2 . We previously observed that the 2Kidney-1Clip hypertension model elicits physical exercise and gastrointestinal dysmotility, which is prevented by renin-angiotensin system blockers. Here, we investigate the effect of physical exercise on inflammation, stress biomarkers, and angiotensin II receptors in the duodenum of 2K1C rats. Methods: Arterial hypertension was induced by the 2K1C surgical model. The rats were allocated in Sham, 2K1C, or 2K1C+Exercise groups. One week after surgery, they were submitted to a physical exercise protocol (running 5x/week, 60min/day). Next, we assessed their intestinal contractility, cytokine levels (TNF-α, IL-1β, and IL-6), oxidative stress levels (MPO, GSH, MDA, and SOD), and the gene expression of angiotensin receptors (ATR 1A , ATR 1B , and ATR 2 ). Results: In comparison with the Sham group, the 2K1C arterial hypertension decreased ( p <0.05) the intestinal contractility. In comparison with 2K1C, the 2K1C+Exercise group exhibited lower ( p <0.05) MPO activity (22.04±5.90 vs. 78.95±18.09 UMPO/mg tissue) and higher ( p <0.05) GSH concentrations in intestinal tissues (67.63±7.85 vs. 31.85±5.90mg NPSH/mg tissue). The 2K1C+Exercise group showed lower ( p <0.05) cytokine levels in the intestine than 2K1C rats. In comparison with the Sham group, the 2K1C+Exercise rats showed higher ( p <0.05) gene expression of ATR 2 in the duodenum. Conclusion: 2K-1C hypertension elicits an oxidative stress and inflammation process in the duodenum. Physical exercise modulates the expression twice as much of ATR2 receptors, suggesting possible anti-inflammatory and antioxidant effects induced by exercise.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 da Silva, Severo, dos Santos, Mendes, Nobre, de Oliveira, Ferreira, Medeiros, Lima-Junior, Havt, Palheta-Junior, dos Santos and Tolentino.)
Databáze: MEDLINE
Externí odkaz: