2-Dodecylmalonic acid-mediated synthesis of mineralized hydroxyapatite amicable for bone cell growth on orthopaedic implant.

Autor: Mullick P; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India., Das G; Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: gdas@iitg.ac.in., Aiyagari R; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: aramesh@iitg.ac.in.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2022 Feb 15; Vol. 608 (Pt 3), pp. 2298-2309. Date of Electronic Publication: 2021 Oct 27.
DOI: 10.1016/j.jcis.2021.10.157
Abstrakt: The present study illustrates the use of 2-dodecylmalonic acid (MA) as a template in biomineralization-inspired synthesis of hydroxyapatite nanoparticles (HANPs). HANPs synthesized in presence of various concentrations of MA displayed varying particle size and shape. The smallest particle size (22-27 nm) was obtained for MA 2 -HANP synthesized in presence of 37 µM MA. The critical micelle concentration (CMC) for MA at pH 9.0 relevant for mineralization was ∼35 µM. AFM analysis revealed that at a low concentration of 10 µM and pH 9.0, MA could generate oblong-shaped aggregates. At 40 µM, comparable to the concentration used to generate MA 2 -HANP, the amphiphile self-assembled to form a spherical soft scaffold, which likely regulated spatial confinement of ions during mineralization and generated small size HANPs. Osteoblast-like MG-63 cells seeded on titanium wire (TW) coated with MA 2 -HANP-incorporated collagen type I (H-TW) displayed enhanced cell proliferation, high expression of osteogenic differentiation marker genes (Col I, ALP, OCN and Runx2) and copious calcium mineral deposition after 14 days of growth. The nuanced role of the self-assembly process of an amphiphilic template in HANP mineralization unravelled in the present study can guide future scaffold design for biomineralization-inspired synthesis of HANPs tailored for bone tissue engineering applications.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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