MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens.

Autor: Liu ZZG; Department of Pathology and Molecular Medicine, McMaster Health Sciences Center, Hamilton, ON L8N 3Z5, Canada., Taiyab A; Department of Pathology and Molecular Medicine, McMaster Health Sciences Center, Hamilton, ON L8N 3Z5, Canada., West-Mays JA; Department of Pathology and Molecular Medicine, McMaster Health Sciences Center, Hamilton, ON L8N 3Z5, Canada.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2021 Nov 05; Vol. 22 (21). Date of Electronic Publication: 2021 Nov 05.
DOI: 10.3390/ijms222111988
Abstrakt: Fibrotic cataracts have been attributed to transforming growth factor-beta (TGF-β)-induced epithelial-to-mesenchymal transition (EMT). Using mouse knockout (KO) models, our laboratory has identified MMP9 as a crucial protein in the TGF-β-induced EMT process. In this study, we further revealed an absence of alpha-smooth muscle actin (αSMA) and filamentous-actin (F-actin) stress fibers in MMP9KO mouse lens epithelial cell explants (LECs). Expression analysis using NanoString revealed no marked differences in αSMA ( ACTA2 ) and beta-actin (β-actin) ( ACTB ) mRNA between the lenses of TGF-β-overexpressing (TGF-βtg) mice and TGF-βtg mice on a MMP9KO background. We subsequently conducted a protein array that revealed differential regulation of proteins known to be involved in actin polymerization and cell migration in TGF-β-treated MMP9KO mouse LECs when compared to untreated controls. Immunofluorescence analyses using rat LECs and the novel MMP9-specific inhibitor, JNJ0966, revealed similar differential regulation of cortactin, FAK, LIMK1 and MLC2 as observed in the array. Finally, a reduction in the nuclear localization of MRTF-A, a master regulator of cytoskeletal remodeling during EMT, was observed in rat LECs co-treated with JNJ0966 and TGF-β. In conclusion, MMP9 deficiency results in differential regulation of proteins involved in actin polymerization and cell migration, and this in turn prevents TGF-β-induced EMT in the lens.
Databáze: MEDLINE
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