| Autor: |
Surówka E; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Latowski D; Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian University, ul. Gronostajowa 7, 30-387 Kraków, Poland., Dziurka M; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Rys M; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Maksymowicz A; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Żur I; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Olchawa-Pajor M; Department of Environmental Protection, Faculty of Mathematics and Natural Sciences, University of Applied Sciences in Tarnow, Mickiewicza 8, 33-100 Tarnów, Poland., Desel C; Botanical Institute of the Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany., Krzewska M; The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland., Miszalski Z; W. Szafer Institute of Botany, Polish Academy of Sciences, ul. Lubicz 46, 31-512 Kraków, Poland. |
| Abstrakt: |
To determine the role of α- and γ-tocopherol (TC), this study compared the response to salt stress (200 mM NaCl) in wild type (WT) Arabidopsis thaliana (L.) Heynh. And its two mutants: (1) totally TC-deficient vte1 ; (2) vte4 accumulating γ-TC instead of α-TC; and (3) tmt transgenic line overaccumulating α-TC. Raman spectra revealed that salt-exposed α-TC accumulating plants were more flexible in regulating chlorophyll, carotenoid and polysaccharide levels than TC deficient mutants, while the plants overaccumulating γ-TC had the lowest levels of these biocompounds. Tocopherol composition and NaCl concentration affected xanthophyll cycle by changing the rate of violaxanthin de-epoxidation and zeaxanthin formation. NaCl treated plants with altered TC composition accumulated less oligosaccharides than WT plants. α-TC deficient plants increased their oligosaccharide levels and reduced maltose amount, while excessive accumulation of α-TC corresponded with enhanced amounts of maltose. Salt-stressed TC-deficient mutants and tmt transgenic line exhibited greater proline levels than WT plants, lower chlorogenic acid levels, and lower activity of catalase and peroxidases. α-TC accumulating plants produced more methylated proline- and glycine- betaines, and showed greater activity of superoxide dismutase than γ-TC deficient plants. Under salt stress, α-TC demonstrated a stronger regulatory effect on carbon- and nitrogen-related metabolites reorganization and modulation of antioxidant patterns than γ-TC. This suggested different links of α- and γ-TCs with various metabolic pathways via various functions and metabolic loops. |
