| Autor: |
Dehshahri A; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 7146864685, Iran., Biagioni A; Department of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, 50134 Florence, Italy., Bayat H; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717443, Iran.; Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115111, Iran., Lee EHC; Cancer Science Institute of Singapore, National University of Singapore, Singapore 637551, Singapore.; Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 637551, Singapore., Hashemabadi M; Department of Biology, Faculty of Sciences, Shahid Bahonar University, Kerman 7616914111, Iran., Fekri HS; Student Research Committee, Kerman University of Medical Sciences, Kerman 7619813159, Iran.; Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman 7616911319, Iran., Zarrabi A; Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Sariyer, Istanbul 34396, Turkey., Mohammadinejad R; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman 7619813159, Iran., Kumar AP; Cancer Science Institute of Singapore, National University of Singapore, Singapore 637551, Singapore.; Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 637551, Singapore.; NUS Centre for Cancer Research (N2CR), Yong Loo Lin School of Medicine, National University of Singapore, Singapore 637551, Singapore. |
| Abstrakt: |
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and its associated proteins (Cas) is an adaptive immune system in archaea and most bacteria. By repurposing these systems for use in eukaryote cells, a substantial revolution has arisen in the genome engineering field. In recent years, CRISPR-Cas technology was rapidly developed and different types of DNA or RNA sequence editors, gene activator or repressor, and epigenome modulators established. The versatility and feasibility of CRISPR-Cas technology has introduced this system as the most suitable tool for discovering and studying the mechanism of specific genes and also for generating appropriate cell and animal models. SOX genes play crucial roles in development processes and stemness. To elucidate the exact roles of SOX factors and their partners in tissue hemostasis and cell regeneration, generating appropriate in vitro and in vivo models is crucial. In line with these premises, CRISPR-Cas technology is a promising tool for studying different family members of SOX transcription factors. In this review, we aim to highlight the importance of CRISPR-Cas and summarize the applications of this novel, promising technology in studying and decoding the function of different members of the SOX gene family. |
